Protein phosphorylation during phagosome maturation

FEBS Letters
N EmansMichel Desjardins

Abstract

Phagolysosome biogenesis is driven by a series of interactions between phagosomes and organelles of the biosynthetic and endocytic pathways. The presence of endocytic markers on phagosomes suggests that phagosomes and endosomes share common structural and functional characteristics. In that line of thought, protein phosphorylation has been shown to be involved in regulatory aspects of the fusion properties of endosomes and other vacuolar organelles. To study further the mechanisms involved in phagolysosome biogenesis, we have investigated the presence of phagosome proteins that can be phosphorylated in vitro by endogenous phagosome-associated kinases. The results obtained show that proteins phosphorylated on tyrosine residues are present on phagosomes. Moreover, complex phosphorylation/dephosphorylation cycles appear to occur during phagolysosome biogenesis. The addition of endosome fractions to phagosomes inhibit the phosphorylation of phagosome proteins. These results suggest that phosphorylation and dephosphorylation events could play roles in the biogenesis of phagolysosomes and regulate, in part, the complex in vivo interactions between phagosomes and endosomes.

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Citations

Apr 4, 1998·Electrophoresis·S ScianimanicoM Desjardins
Apr 16, 2002·Molecular Biology of the Cell·Hélène DefacqueGareth Griffiths
Jan 19, 2000·The EMBO Journal·H DefacqueG Griffiths
Dec 9, 2008·BMC Systems Biology·Mark KühnelJens Reich
May 10, 2006·Cellular Microbiology·Elsa AnesGareth Griffiths
Jul 13, 2004·Trends in Cell Biology·Gareth Griffiths
Jun 16, 1997·The Journal of Experimental Medicine·M Desjardins, A Descoteaux
Dec 15, 2006·Journal of Cellular Physiology·Kousuke KasaharaNaoto Yamaguchi
Nov 7, 1998·The Journal of Biological Chemistry·A JahrausG Griffiths
Sep 16, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pearl GrayMoshe Arditi
Nov 25, 2003·European Journal of Cell Biology·Katharina von LöhneysenMarkus Maniak

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