PMID: 2498290Jun 1, 1989Paper

Protein phosphorylation in Bradyrhizobium japonicum bacteroids and cultures

Journal of Bacteriology
D B Karr, D W Emerich

Abstract

Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling patterns and intensities of in vivo- and in vitro-labeled phosphoproteins showed a number of similarities; however, several phosphoproteins were found only after one of the two labeling conditions. The labeling intensity was time dependent in both in vivo and in vitro assays and was dependent on the presence of magnesium in in vitro assays. Differences in the rates of phosphorylation and dephosphorylation were noted for a number of proteins. The level of incorporation of 32P into protein was only 2% or less of the total phosphate accumulated during the in vivo labeling period. Several isolation and sample preparation procedures resulted in differences in labeling patterns. Phosphatase inhibitors and several potential metabolic effectors had negligible effects on the phosphorylation pattern. There were no significant changes in the phosphorylation patterns of cells cultured on mannitol, acetate, and succinate, alth...Continue Reading

References

Mar 16, 1979·Science·M Garnak, H C Reeves
Jan 1, 1975·Journal of General Microbiology·C M Brown, M J Dilworth
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F J HanusH J Evans
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W ZilligM Hirsch-Kauffmann
Jan 1, 1988·Annual Review of Microbiology·A J Cozzone
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J Ninfa, B Magasanik
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J F HessM I Simon
Jan 31, 1985·Biochemical and Biophysical Research Communications·R H VallejosM Torruella
Jan 1, 1974·Molecular & General Genetics : MGG·H PontaM Schweiger
Jul 15, 1982·Analytical Biochemistry·M Manai, A J Cozzone
Mar 1, 1983·Journal of Bacteriology·C F McAllister, J E Lepo
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R L HuganirP Greengard

❮ Previous
Next ❯

Citations

Jan 1, 1993·Journal of Cellular Biochemistry·A J Cozzone
Jul 1, 1990·Applied and Environmental Microbiology·I A BalodimosE R Kashket

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.