Protein phosphorylation in meiotically competent and incompetent mouse oocytes.

Molecular Reproduction and Development
E A BornslaegerR M Schultz

Abstract

Specific changes in the two-dimensional gel electrophoretic pattern of mouse oocyte phosphoproteins precede germinal vesicle breakdown (GVBD). We report that changes in the relative abundance of phosphoamino acids occurred prior to GVBD. We also report data that further strengthen the close association of the changes in phosphoprotein patterns with resumption of meiosis. The calmodulin antagonist W7, which transiently inhibits GVBD, inhibited partially at least two of the maturation-associated phosphoprotein changes, the dephosphorylation of a 60,000 Mr phosphoprotein and the phosphorylation of a 36,000 Mr protein. In oocytes from juvenile mice that were incompetent to resume meiosis, neither these changes nor the phosphorylation of proteins of Mr 24,000 and 28,000 occurred; all these changes occurred, however, in oocytes from juvenile mice that were competent to resume meiosis. The microinjection of the heat-stable inhibitor of cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKI), which induces GVBD in fully grown oocytes, did not induce GVBD in meiotically incompetent oocytes. Microinjected PKI did not induce the increased protein phosphorylations associated with maturation, but it did induce the dephosphoryla...Continue Reading

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Citations

Dec 1, 1993·Molecular Reproduction and Development·L GallV De Smedt
Jan 1, 1995·Molecular Reproduction and Development·S T Homa
Jul 1, 1992·Developmental Biology·D Wickramasinghe, D F Albertini
Jun 29, 2002·Molecular and Cellular Endocrinology·M KovoN Dekel
Nov 1, 1995·Zygote : the Biology of Gametes and Early Embryos·Y HiraoR M Moor
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J Van Blerkom
Jul 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Michal KovoNava Dekel
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Catherine LedentGilbert Vassart
Jun 23, 2000·Developmental Biology·R Czołowska, E Borsuk
Dec 1, 1993·The Journal of Experimental Zoology·Y HiraoS Kato
Dec 14, 2004·Science·Lisa M MehlmannLaurinda A Jaffe

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