PMID: 7544316Aug 15, 1995Paper

Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium

Genes & Development
P C Ostrovsky, S Maloy

Abstract

There exists a plethora of tyrosine kinases that play essential roles in regulation of eukaryotic proteins. Several dual specificity kinases that phosphorylate proteins on threonine, serine, and tyrosine residues also play critical roles in eukaryotic phosphorylation cascades. In contrast, very few prokaryotic proteins have been shown to be phosphorylated on tyrosine residues, and the functions of the rare examples remain obscure. Furthermore, no dual specificity kinases have been described in prokaryotes. Our results indicate that PutA protein from the bacterium Salmonella typhimurium autophosphorylates on several threonine, serine, and tyrosine residues. PutA protein both represses the proline utilization (put) operon and degrades proline to glutamate. These two opposing functions are regulated by the availability of proline and the membrane sites needed for the proline dehydrogenase activity of PutA protein. In addition, these functions are modulated by phosphorylation of PutA protein. The rate of dephosphorylation of PutA protein is determined by the availability of proline and membranes. Dephosphorylated PutA protein has a higher DNA binding affinity than the phosphorylated protein and thus may prevent toxic overexpression...Continue Reading

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Citations

Mar 6, 1999·Current Opinion in Microbiology·G H Kelemen, M J Buttner
Mar 6, 2012·International Journal of Oral Science·Sarah E Whitmore, Richard J Lamont
May 25, 2004·The Journal of Biological Chemistry·Dan GuDonald F Becker
Aug 15, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christophe GrangeasseIvan Mijakovic
Dec 25, 2004·Infection and Immunity·Vijayakrishna SinguRoman R Ganta
Jul 14, 2000·Journal of Bacteriology·S RashkovaP J Christie
Jul 5, 2005·Journal of Bacteriology·Lucia MusumeciNunzio Bottini
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J WuA Newton
Jan 9, 2007·Trends in Biochemical Sciences·Christophe GrangeasseIvan Mijakovic
Jul 3, 2019·Journal of Bacteriology·Landon J GetzNikhil A Thomas
Feb 21, 2014·The Journal of Biological Chemistry·Joseph D ChaoYossef Av-Gay
Jan 12, 1999·Microbiology·P FreestoneV Norris
Jun 27, 1998·Journal of Molecular Biology·P FreestoneV Norris
Apr 1, 1997·Journal of Bacteriology·B McCartneyM Potts
Apr 1, 1997·Journal of Bacteriology·S C SmithM Potts
Nov 1, 2005·EcoSal Plus·Larry Reitzer

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