Protein-polyelectrolyte complexes to improve the biological activity of proteins in layer-by-layer assemblies

Nanoscale
A Vander StraetenC Dupont-Gillain

Abstract

A standard method of protein immobilization is proposed, based on the use of protein-polyelectrolyte complexes (PPCs) as building blocks for layer-by-layer assembly. Thicker multilayers, with a higher polyelectrolyte fraction, are obtained with PPCs compared to single protein molecules. Biological activity is not only maintained, but specific activity is also higher, as demonstrated for lysozyme-poly(styrene sulfonate) complexes. This is attributed to the more hydrated state of the assemblies. This new method of protein immobilization opens up perspectives for biotechnology and biomedical applications.

References

Mar 6, 2003·Journal of the American Chemical Society·Fredrik CarlssonPer Linse
May 26, 2005·Journal of Colloid and Interface Science·Jing SunZhiqiang Wang
Oct 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Fabrice CousinFrançois Boué
Oct 20, 2005·Journal of Bioscience and Bioengineering·M OndaT Kunitake
Mar 22, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Oliver Hollmann, Claus Czeslik
Nov 21, 2007·Colloids and Surfaces. B, Biointerfaces·Willem Norde
Jul 11, 2008·Macromolecular Bioscience·Katsuhiko ArigaQingmin Ji
Dec 25, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Yongmei GuoJunqi Sun
Aug 3, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Rubens A SilvaPaul L Dubin
Dec 25, 2012·Colloids and Surfaces. B, Biointerfaces·Anna Bratek-SkickiŻbigniew Adamczyk
Aug 21, 2014·Chemical Reviews·João Borges, João F Mano
May 11, 2017·Accounts of Chemical Research·Christian DoonanPaolo Falcaro

❮ Previous
Next ❯

Citations

Mar 13, 2020·Biomaterials·Apoorva SarodeSamir Mitragotri
Dec 21, 2020·Advances in Colloid and Interface Science·Joëlle Bizeau, Damien Mertz
Aug 2, 2018·Biomacromolecules·Lydia KisleyDeborah E Leckband
Jul 3, 2018·ACS Nano·Aurélien Vander StraetenChristine Dupont-Gillain
Jul 30, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Yaqian CaiHanying Zhao
Jun 28, 2018·The Journal of Physical Chemistry. B·Aristeidis PapagiannopoulosJudith Elizabeth Houston
Jan 1, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Aurélien Vander Straeten, Christine Dupont-Gillain
Jul 16, 2020·Chemical Reviews·Romaric GérardyJean-Christophe M Monbaliu
Jan 15, 2021·The Journal of Physical Chemistry Letters·Vincent DelmezArnaud Delcorte

❮ Previous
Next ❯

Methods Mentioned

BETA
QCM-D
atomic force microscopy
AFM
fluorescence spectrometry

Software Mentioned

CONTIN

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Related Papers

Journal of Biomedical Materials Research
D W MarshallH J Bixler
Methods in Cell Biology
H F Epstein, F Liu
MMW Fortschritte der Medizin
Marion Hofmann-Aßmus
© 2022 Meta ULC. All rights reserved