Protein post-translational modification in host defense: the antimicrobial mechanism of action of human eosinophil cationic protein native forms

The FEBS Journal
Vivian A SalazarEster Boix

Abstract

Knowledge on the contribution of protein glycosylation in host defense antimicrobial peptides is still scarce. We have studied here how the post-translational modification pattern modulates the antimicrobial activity of one of the best characterized leukocyte granule proteins. The human eosinophil cationic protein (ECP), an eosinophil specific granule protein secreted during inflammation and infection, can target a wide variety of pathogens. Previous work in human eosinophil extracts identified several ECP native forms and glycosylation heterogeneity was found to contribute to the protein biological properties. In this study we analyze for the first time the antimicrobial activity of the distinct native proteins purified from healthy donor blood. Low and heavy molecular weight forms were tested on Escherichia coli cell cultures and compared with the recombinant non-glycosylated protein. Further analysis on model membranes provided an insight towards an understanding of the protein behavior at the cytoplasmic membrane level. The results highlight the significant reduction in protein toxicity and bacteria agglutination activity for heavy glycosylated fractions. Notwithstanding, the lower glycosylated fraction mostly retains the l...Continue Reading

References

Dec 1, 1989·The Journal of Experimental Medicine·T L WasmoenGerald J Gleich
May 1, 1988·European Journal of Haematology·C PetersonPer Venge
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Gerald J GleichD J McKean
Jul 1, 1985·The American Journal of Tropical Medicine and Hygiene·S J AckermanA E Butterworth
Sep 1, 1994·Protein Science : a Publication of the Protein Society·William C WimleyS H White
Oct 7, 1998·Cellular and Molecular Life Sciences : CMLS·J J Beintema, R G Kleineidam
Sep 1, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Per VengeAgneta Trulson
Mar 28, 2001·Science·A Helenius, M Aebi
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·U-B JönssonPer Venge
Feb 27, 2004·Biochemical and Biophysical Research Communications·Byung-Moon KimYounghoon Lee
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bin YeDaniel W Cramer
Jul 11, 2006·Current Opinion in Biotechnology·Natarajan Sethuraman, Terrance A Stadheim
Jan 26, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Agneta TrulsonPer Venge
May 12, 2007·Glycobiology·Karin Julenius
Aug 5, 2008·Current Pharmaceutical Biotechnology·Helene F Rosenberg
Aug 13, 2008·The Journal of Biological Chemistry·Martina UlrichGerd Döring
Oct 11, 2008·Nature Reviews. Immunology·Jamey D Marth, Prabhjit K Grewal
Feb 28, 2009·Nature Reviews. Drug Discovery·Roy Jefferis
May 2, 2009·Molecular and Biochemical Parasitology·James P HewitsonRick M Maizels
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenny RubinPer Venge
Aug 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charlotte WoschnaggPer Venge
Sep 1, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Douglas A PlagerKristin M Leiferman
Jun 2, 2010·Biophysical Journal·María Flor García-MayoralMarta Bruix
Nov 3, 2010·Cell and Tissue Research·Revital ShamriLisa A Spencer
Nov 30, 2010·Cell·Gerald W Hart, Ronald J Copeland
Jan 14, 2011·Journal of Innate Immunity·Praveen AkuthotaPeter F Weller
Jan 18, 2011·Respiratory Research·Jonas BystromDavid Bishop-Bailey
Jun 18, 2011·Experimental Hematology·Ingrid GlimeliusGunilla Enblad
Jul 5, 2011·Glycobiology·Simon J NorthStuart M Haslam
Dec 7, 2011·ACS Chemical Biology·Lai-Xi Wang, Joseph V Lomino
Jan 10, 2012·Current Opinion in Chemical Biology·Daniel KolarichPeter H Seeberger
Jan 14, 2012·Critical Reviews in Microbiology·Anu Malik, Janendra K Batra
Jun 27, 2012·Journal of the American Chemical Society·Aram J KrausonWilliam C Wimley
Sep 5, 2012·Biological Chemistry·Ester BoixMohammed Moussaoui
Nov 17, 2012·Nature Reviews. Immunology·Helene F RosenbergPaul S Foster
May 30, 2013·Antimicrobial Agents and Chemotherapy·David PulidoEster Boix
Jun 27, 2013·Virulence·Anne Marie Krachler, Kim Orth

Citations

Mar 25, 2016·International Journal of Molecular Sciences·Brian Becknell, John David Spencer
Jun 21, 2017·Molecular & Cellular Proteomics : MCP·Ian LokeMorten Thaysen-Andersen
May 6, 2019·RNA·Emily R GarnettRonald T Raines
Mar 18, 2020·Current Topics in Medicinal Chemistry·Marina KurbasicSilvia Marchesan
Jun 6, 2018·Frontiers in Immunology·Lu LuEster Boix
Oct 29, 2020·Molecular Biology Reports·Benudhar MukhiSusanta Kumar Ghosh

Related Concepts

RNASE3 protein, human
Alkalescens-Dispar Group
Protein Glycosylation
Fungus Drug Sensitivity Tests
Post-Translational Protein Processing
Eosinophil cationic protein
Agglutination
Cations
Cell Culture Techniques
Plasma Membrane

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.