Protein production, crystallization and preliminary X-ray analysis of two isoforms of the Dscam1 Ig7 domain

Acta Crystallographica. Section F, Structural Biology Communications
Shu-Ang LiQiang Chen

Abstract

Drosophila Down syndrome cell adhesion molecule 1 (Dscam1) plays a critical role in neural development. It can potentially form 38 016 isoforms through alternative RNA splicing, and exhibits isoform-specific homophilic interaction through three variable Ig domains (Ig2, Ig3 and Ig7). The diversity and homophilic interaction are essential for its functions. Ig7 has 33 isoforms and is the most variable among the three variable Ig domains. However, only one isoform of Ig7 (isoform 30) has been structurally determined to date. Here, two isoforms of Dscam1 Ig7 (isoforms 5 and 9; Ig75 and Ig79) were produced and crystallized. Diffraction data from Ig75 and Ig79 crystals were processed to resolutions of 1.95 and 2.37 Å, respectively. Comparison of different Dscam1 Ig7 isoforms will provide insight into the mechanism of its binding specificity.

References

Aug 20, 2005·Science·Fiona L WatsonDietmar Schmucker
May 8, 2007·Cell·Benjamin J MatthewsWesley B Grueber
Sep 14, 2007·Nature·Daisuke HattoriBarry J Dickson
Apr 12, 2008·Molecular Biology and Evolution·Daniela BritesLouis Du Pasquier
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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