Protein profiling of cervical cancer by protein-biochips: proteomic scoring to discriminate cervical cancer from normal cervix

Cancer Letters
Y F WongS C Mok

Abstract

Analysis of multiple proteins is thought to be essential for establishment of signature proteomic patterns that may distinguish cancer from non-cancer. Surface-enhanced laser desorption/ionization (SELDI) is an affinity-based mass spectrometric method in which proteins of interest are selectively absorbed to a chemically modified surface on a biochip. This technology may provide protein profiling of a variety of biological specimens. In this study, we explored whether the protein biochip SELDI approach could differentiate cervical cancer from non-cancer cohorts. We screened protein profiles generated by SELDI in 62 cervical epithelial cell samples microdissected from 35 invasive cervical cancer and 27 age-matched normal cervix tissue specimens, respectively. The cell lysates of pure populations of cervical cells were applied onto Ciphergen ProteinChip WCX2 Arrays. Proteins bound to the chips were analyzed on a ProteinChip Reader Model PBS II. Derived proteomic patterns were converted to a simple proteomic scoring for distinguishing cancer from non-cancer cohorts. SELDI protein profiles of cell lysates from 20 cervical cancer and 15 normal cervix tissue specimens were used to train and develop a classification scoring system tha...Continue Reading

Citations

May 2, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Jesús García-FoncillasNatalia Remírez
Jan 13, 2006·Cancer Immunology, Immunotherapy : CII·Amanda K MilesRobert C Rees
Jan 13, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Leticia SantosRamón Gerardo Guevara-González
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Mandy G Katz-JaffeDavid K Gardner
May 17, 2011·Cellular Oncology (Dordrecht)·Kai-Erik UlebergJan P A Baak
Dec 19, 2006·Critical Reviews in Clinical Laboratory Sciences·Catherine J LeeDavid A Fishman
Jun 15, 2006·Expert Review of Proteomics·Jérôme SolassolAlain Mangé
Dec 10, 2008·Journal of Proteomics·Gitika PanickerElizabeth R Unger
Nov 5, 2008·Fertility and Sterility·Stephanie J EstesStacey A Missmer
Nov 15, 2008·Gynecologic Oncology·Xueqiong ZhuShanshan Jiang
Nov 5, 2005·Pediatric Transplantation·Avram Z Traum, Asher D Schachter
Feb 13, 2008·Journal of Cellular and Molecular Medicine·L C WhelanW M Gallagher
Mar 7, 2014·IEEE Transactions on Nanobioscience·Chen Liao, Shiyan Hu
Jan 25, 2005·Molecular and Cellular Endocrinology·Zhen XiaoHaleem J Issaq
May 16, 2006·Gastroenterology·Christopher J ScarlettRobert C Baxter
Jan 18, 2006·Fertility and Sterility·Mandy G Katz-JaffeWilliam B Schoolcraft
Oct 6, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·A PreethaR Banerjee
Sep 13, 2014·PloS One·Geert A A Van RaemdonckXaveer W M Van Ostade
Feb 1, 2006·Expert Review of Proteomics·Eun-Kyoung Yim, Jong-Sup Park
Mar 4, 2006·Expert Review of Molecular Diagnostics·Cara M MartinJohn J O'Leary
Dec 16, 2005·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·N A Henderson, R J C Steele
Sep 30, 2004·Technology in Cancer Research & Treatment·M Verma
Sep 15, 2006·Proteomics·Yasuhiro Kuramitsu, Kazuyuki Nakamura
Oct 1, 2014·The British Journal of General Practice : the Journal of the Royal College of General Practitioners·Fiona M WalterRichard D Neal
Sep 22, 2018·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Vian H RasulMohhammad A Cheraghi
Apr 16, 2005·Clinical Science·Walter KolchAndrew R Pitt
Sep 23, 2006·Clinical Chemistry·Ali BouamraniFrançois Berger
Aug 24, 2005·Médecine sciences : M/S·Jérôme SolassolAlain Mangé
Oct 30, 2007·Molecular Diagnosis & Therapy·Cara M MartinJohn J O'Leary
Mar 15, 2006·BioTechniques·DaRue A PrietoDavid B Krizman
Jul 20, 2007·Chemical Reviews·Haleem J IssaqTimothy D Veenstra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.