Protein profiling of microdissected prostate tissue links growth differentiation factor 15 to prostate carcinogenesis

Cancer Research
Peter K CheungJuergen Vielkind

Abstract

Identification of proteomic alterations associated with early stages in the development of prostate cancer may facilitate understanding of progression of this highly variable disease. Matched normal, high-grade prostatic intraepithelial neoplasia (hPIN) and prostate cancer cells of predominantly Gleason grade 3 were procured by laser capture microdissection from serial sections obtained from snap-frozen samples dissected from 22 radical prostatectomy specimens. From these cells, protein profiles were generated by surface-enhanced laser desorption/ionization-time of flight mass spectrometry. A 24-kDa peak was observed at low or high intensity in profiles of prostate cancer cells in 19 of 27 lesions and at low intensity in 3 of 8 hPIN lesions but was not detectable in matched normal cells. SDS-PAGE analysis of prostate cancer and matched normal epithelium confirmed expression of a prostate cancer-specific 24-kDa protein. Mass spectrometry and protein data-based analysis identified the protein as the dimeric form of mature growth differentiation factor 15 (GDF15). The increased expression of mature GDF15 protein in prostate cancer cells cannot be explained on the basis of up-regulation of GDF15 mRNA because reverse transcription-P...Continue Reading

References

Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·M R BootcovS N Breit
Dec 31, 1997·Biochimica Et Biophysica Acta·R HromasL Lu
Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
May 24, 2003·Biochemical and Biophysical Research Communications·Dev KaranSurinder K Batra
Aug 2, 2003·International Journal of Surgical Pathology·Kenneth A Iczkowski, Cooley G Pantazis
Nov 20, 2003·Mass Spectrometry Reviews·Ning TangScot R Weinberger
Dec 12, 2003·Cancer·Yaxin ZhengBrian C-S Liu

❮ Previous
Next ❯

Citations

Apr 3, 2010·Der Urologe. Ausg. A·D WittschieberA Erbersdobler
Aug 11, 2007·World Journal of Urology·Eric Schiffer
Jun 1, 2006·Virchows Archiv : an International Journal of Pathology·Manfred Dietel, Christine Sers
May 1, 2008·Journal of Cancer Research and Clinical Oncology·Qiaojia HuangXu Lin
Jun 22, 2013·Cytokine & Growth Factor Reviews·Klaus UnsickerKerstin Krieglstein
Apr 9, 2005·Current Opinion in Urology·Lionel L BañezJudd W Moul
Jun 25, 2005·Journal of Clinical Pathology·C HughesJ O'Leary
Nov 28, 2007·Annual Review of Pathology·Scott A TomlinsArul M Chinnaiyan
Feb 24, 2007·Vascular Health and Risk Management·Mitsuko FuruyaHiroshi Ishikura
Jul 8, 2011·Future Oncology·Katayoon Kasaian, Steven Jm Jones
Mar 5, 2014·Asian Journal of Andrology·Yan Ting ChiangYuzhuo Wang
Jan 15, 2014·Cancer Metastasis Reviews·Rhonda L BittingAndrew J Armstrong
Oct 20, 2006·Critical Reviews in Clinical Laboratory Sciences·Qinghua FengNancy B Kiviat
Nov 26, 2010·Scandinavian Journal of Urology and Nephrology·Sara Jonmarker JarajLars Egevad
Nov 3, 2010·Scandinavian Journal of Urology and Nephrology·Lars HäggarthLars Egevad
Jul 2, 2011·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Xinye XuWei Gao
Dec 26, 2012·Clinical Biochemistry·Elisa PinEmanuel F Petricoin
Jan 6, 2007·Journal of Proteome Research·Christian MelleFerdinand von Eggeling
Aug 5, 2006·The Journal of Investigative Dermatology·Guang YangHensin Tsao
Jan 29, 2009·International Journal of Cancer. Journal International Du Cancer·Stephanie MaKwok Wah Chan
Feb 1, 2009·Proteomics. Clinical Applications·Caroline A EvansIshtiaq Rehman
Jan 4, 2012·Histopathology·Karen S Sfanos, Angelo M De Marzo
Jun 26, 2010·Journal of Cellular Physiology·Murielle Mimeault, Surinder K Batra
Mar 19, 2016·Journal of Cellular and Molecular Medicine·Chen LiHonghe Zhang
Feb 9, 2007·Differentiation; Research in Biological Diversity·Samina NooraliJuergen Vielkind
Jun 2, 2006·Molecular & Cellular Proteomics : MCP·Nilofer S AzadElise C Kohn
Feb 8, 2005·Molecular & Cellular Proteomics : MCP·Michael E WrightRuedi Aebersold
Aug 11, 2005·Molecular & Cellular Proteomics : MCP·Brian L HoodDavid B Krizman
May 10, 2018·Andrologia·M B FrungieriM E Matzkin
Jun 16, 2006·BioTechniques·Thomas P ConradsTimothy D Veenstra
Jul 20, 2007·Proteomics·Ferdinand von EggelingGünther Ernst
Sep 1, 2007·Proteomics. Clinical Applications·Magnus HellströmLars Egevad
May 18, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Yifei ZhangLeping Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.