Protein profiling of osteosarcoma tissue and soft callus unveils activation of the unfolded protein response pathway

International Journal of Oncology
Parunya ChaiyawatDumnoensun Pruksakorn

Abstract

Oncogenic drivers of osteosarcoma remain controversial due to the complexity of the genomic background of the disease. There are limited novel therapeutic options, and the survival rate of patients with osteosarcoma has not improved in decades. Genomic instability leads to complexity in various pathways, which is potentially revealed at the protein level. Therefore, the present study aimed to identify the mechanisms involved in the oncogenesis of osteosarcoma using proteomics and bioinformatics tools. As clinical specimens from patients are the most relevant disease‑related source, expression patterns of proteins in osteosarcoma tissues were compared with soft tissue callus from donors containing high numbers of osteoblastic cells. Two‑dimensional electrophoresis and liquid chromatography‑tandem mass spectrometry (LC‑MS/MS) successfully identified 33 differentially expressed proteins in the osteosarcoma tissues compared with the soft tissue callus. Among these proteins, 29 proteins were significantly upregulated in osteosarcoma. A functionally grouped network of the overexpressed proteins, that was created using the ClueGo and CluePedia applications, demonstrated that the unfolded protein response (UPR) pathway was activated ma...Continue Reading

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Methods Mentioned

BETA
biopsy
electrophoresis
PCR
amputation
protein folding

Clinical Trials Mentioned

NCT00027716

Software Mentioned

CluePedia
GraphPad
Mascot
WebGestalt
GraphPad Prism
ClueGO
ImageMaster 2D Platinum
Cytoscape
MassLynx
ProteinLynx

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