Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle

Nature Communications
Youjun ZhangToshihiro Obata

Abstract

Protein complexes of sequential metabolic enzymes, often termed metabolons, may permit direct channelling of metabolites between the enzymes, providing increased control over metabolic pathway fluxes. Experimental evidence supporting their existence in vivo remains fragmentary. In the present study, we test binary interactions of the proteins constituting the plant tricarboxylic acid (TCA) cycle. We integrate (semi-)quantitative results from affinity purification-mass spectrometry, split-luciferase and yeast-two-hybrid assays to generate a single reliability score for assessing protein-protein interactions. By this approach, we identify 158 interactions including those between catalytic subunits of sequential enzymes and between subunits of enzymes mediating non-adjacent reactions. We reveal channelling of citrate and fumarate in isolated potato mitochondria by isotope dilution experiments. These results provide evidence for a functional TCA cycle metabolon in plants, which we discuss in the context of contemporary understanding of this pathway in other kingdoms.

References

Apr 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H O Spivey, J M Merz
Jun 9, 1986·FEBS Letters·S J Barnes, P D Weitzman
Jul 29, 1983·Biochimica Et Biophysica Acta·S F D'Souza, P A Srere
Dec 12, 1983·Biochimica Et Biophysica Acta·Z PorpáczyI Alkonyi
Feb 1, 1995·The International Journal of Biochemistry & Cell Biology·M al-Habori
May 1, 1994·Trends in Biochemical Sciences·G R Welch, J S Easterby
Oct 1, 1993·Journal of Bacteriology·C K Mathews
Oct 29, 1998·The Journal of Biological Chemistry·I Morgunov, P A Srere
Oct 21, 1999·Methods : a Companion to Methods in Enzymology·H O Spivey, J Ovádi
Mar 1, 2000·Trends in Biochemical Sciences·P A Srere
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·M Laloi
Apr 6, 2001·Metabolic Engineering·W van WindenS Heijnen
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Oct 9, 2002·The Plant Cell·Mireia PanicotAntonio F Tiburcio
Apr 4, 2003·The Journal of Biological Chemistry·Michael J ConsidineLee J Sweetlove
Jul 13, 2004·Bio Systems·Daniela DegenringAdelinde M Uhrmacher
Dec 18, 2004·Plant Molecular Biology·A Harvey MillarHans-Peter Braun
Dec 25, 2004·Plant Physiology·Pablo LeivarXavier Fernàndez-Busquets
Feb 24, 2005·Annual Review of Plant Biology·Brenda S J Winkel
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann
Apr 30, 2005·Current Opinion in Plant Biology·Kirsten JørgensenBirger Lindberg Møller
Nov 5, 2005·Plant Molecular Biology·Claudia Studart-GuimarãesFernando Carrari
Sep 12, 2006·Biochimica Et Biophysica Acta·Irina BrandinaRobert P Martin
Feb 23, 2007·The Journal of Biological Chemistry·Mohammad Mainul IslamSusan M Hutson
Jul 31, 2007·The Plant Journal : for Cell and Molecular Biology·Yukichi Fujikawa, Naohiro Kato
Jun 18, 2010·Trends in Plant Science·Lee J SweetloveR George Ratcliffe
Aug 25, 2010·Methods in Molecular Biology·Naohiro Kato, Jason Jones
Dec 4, 2010·Science·Birger Lindberg Møller
Jan 15, 2011·Trends in Plant Science·Jelle Van LeeneLieven De Veylder

❮ Previous
Next ❯

Citations

Nov 6, 2018·The Plant Journal : for Cell and Molecular Biology·Barbara De SmetFrank Van Breusegem
Aug 1, 2019·Journal of the Royal Society, Interface·Florian HinzpeterUlrich Gerland
Sep 15, 2019·The Plant Journal : for Cell and Molecular Biology·Philippe FuchsMarkus Schwarzländer
Nov 13, 2019·Current Protocols in Plant Biology·Youjun ZhangAlisdair R Fernie
Aug 26, 2020·Current Protocols in Plant Biology·Youjun ZhangAlisdair R Fernie
Jun 1, 2018·Nature Communications·Lee J Sweetlove, Alisdair R Fernie
Sep 12, 2018·Annual Review of Microbiology·Stephen K Dolan, Martin Welch
Sep 25, 2019·Research : a Science Partner Journal·Alisdair R FernieLee J Sweetlove
Dec 29, 2019·BMC Plant Biology·Yongming LiuMoju Cao
Jul 12, 2020·Biomolecules·Alisdair R FernieAdriano Nunes-Nesi
Aug 10, 2019·Scientific Reports·Michal GorkaAlexander Graf
Sep 11, 2020·Plant Molecular Biology·Michail MichailidisAthanassios Molassiotis
May 16, 2019·Nature Chemical Biology·Evan M ZhaoJared E Toettcher
May 6, 2020·Antioxidants & Redox Signaling·Youjun Zhang, Alisdair R Fernie
Jan 14, 2021·EMBO Reports·Youjun Zhang, Alisdair R Fernie
Mar 12, 2021·Plant Science : an International Journal of Experimental Plant Biology·Huina ZhaoJuanxu Liu
Apr 2, 2021·Plant Physiology·Mario FenechMiguel A Botella
May 6, 2021·The Plant Genome·Lekha T PazhamalaRajeev K Varshney
Mar 3, 2021·Journal of Experimental Botany·Paula da Fonseca-PereiraWagner L Araújo
May 10, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Nils RugenHolger Eubel
Oct 30, 2021·The Plant Journal : for Cell and Molecular Biology·Manuel BalpardaVeronica G Maurino

❮ Previous
Next ❯

Methods Mentioned

BETA
interaction assay
affinity purification
Y2H
tandem affinity purification
Destination
confocal microscopy

Software Mentioned

Progenesis QI
R
AtGenExpress
SAINT
CRAPome
Mascot
MSCorr
STATIS
Cytoscape

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.