Protein-protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2.

Journal of Experimental Botany
Ewa Lukasik-ShreepaathyFrank L W Takken

Abstract

Plant resistance proteins (R) are involved in pathogen recognition and subsequent initiation of defence responses. Their activity is regulated by inter- and intramolecular interactions. In a yeast two-hybrid screen two clones (I2I-1 and I2I-2) specifically interacting with I-2, a Fusarium oxysporum f. sp. lycopersici resistance protein of the CC-NB-LRR family, were identified. Sequence analysis revealed that I2I-1 belongs to the Formin gene family (SlFormin) whereas I2I-2 has homology to translin-associated protein X (SlTrax). SlFormin required only the N-terminal CC I-2 domain for binding, whereas SlTrax required both I-2 CC and part of the NB-ARC domain. Tomato plants stably silenced for these interactors were not compromised in I-2-mediated disease resistance. When extended or mutated forms of I-2 were used as baits, distinct and often opposite, interaction patterns with the two interactors were observed. These interaction patterns correlated with the proposed activation state of I-2 implying that active and inactive R proteins adopt distinct conformations. It is concluded that the yeast two hybrid system can be used as a proxy to monitor these different conformational states.

References

Dec 1, 1995·The Plant Journal : for Cell and Molecular Biology·A BendahmaneD C Baulcombe
Sep 1, 1996·Trends in Genetics : TIG·H M Duttweiler
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·M RossiV M Williamson
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·J J MesM A Haring
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·F L TakkenM H Joosten
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P Dinesh-KumarB J Baker
Oct 18, 2002·The Plant Journal : for Cell and Molecular Biology·Abdelhafid BendahmaneDavid C Baulcombe
Nov 6, 2002·The Plant Cell·Wladimir I L TamelingBen J C Cornelissen
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Chin-Feng Hwang, Valerie M Williamson
Jul 10, 2003·Molecular Plant-microbe Interactions : MPMI·Gloria NombelaMariano Muñiz
Jan 28, 2005·Molecular Plant-microbe Interactions : MPMI·M RepB J C Cornelissen
Feb 22, 2005·The Plant Cell·Antonious Al-DaoudeMurray Grant
Mar 15, 2005·Nature Cell Biology·Mathieu IngouffFrédéric Berger
Apr 15, 2005·Nature·Stefan J RiedlYigong Shi
Jul 7, 2005·The Plant Journal : for Cell and Molecular Biology·Sergio de la Fuente van BentemBen J C Cornelissen
Oct 11, 2005·The Plant Journal : for Cell and Molecular Biology·Tsutomu KawasakiJeffery L Dangl
Feb 21, 2006·Plant Physiology·Wladimir I L TamelingFrank L W Takken
Mar 31, 2006·Molecular Plant-microbe Interactions : MPMI·Chiyumi ShimadaYoshitaka Takano
May 23, 2006·Current Opinion in Plant Biology·Frank Lw TakkenWladimir Il Tameling
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Dec 19, 2006·Plant Biotechnology Journal·Tadeusz WroblewskiRichard Michelmore
Feb 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jules AdeRoger W Innes
Jun 26, 2007·The Plant Journal : for Cell and Molecular Biology·Tadeusz WroblewskiRichard W Michelmore
Apr 9, 2008·Journal of Experimental Botany·Gerben van OoijenFrank L W Takken
May 10, 2008·PLoS Pathogens·Petra M HoutermanMartijn Rep
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Alexey A TomilovJohn I Yoder
Feb 21, 2009·The Plant Journal : for Cell and Molecular Biology·Petra M HoutermanMartijn Rep

❮ Previous
Next ❯

Citations

Jan 1, 2012·Scientifica·Fatima Cvrčková
Jun 5, 2012·Current Opinion in Plant Biology·Frank L W Takken, Aska Goverse
Jun 15, 2016·Annual Review of Phytopathology·Massimo TurinaRenato O Resende

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY150043
LN836738
AY150044

Methods Mentioned

BETA
nucleotide exchange
two-hybrid
PCR
PCRs
transgenic
phosphotransferase
Y2H
protein folding

Software Mentioned

GraphPad Prism
SignalP
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.