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Protein purification: adsorption chromatography on controlled pore glass with the use of chaotropic buffers

Science

Jan 30, 1976

H G BockS Harshman

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Abstract

Chromatography on controlled pore glass in combination with chaotropic buffers makes possible, in a single step, protein purifications of several hundredfold. The new emphasis is on highly selective controllable adsorption. The method is useful for the purification and concentration of ...read more

Mentioned in this Paper

Soluble
Ampholytes
Phospholipase C
Genus staphylococcus
Buffers
Hydrogen-Ion Concentration
Mitochondrial Membranes
Apoenzymes
Protein Purification
Adsorption
4
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  • Protein purification: adsorption chromatography on controlled pore glass with the use of chaotropic buffers

    Science

    Jan 30, 1976

    H G BockS Harshman

    PMID: 1859

    DOI: 10.1126/science.1859

    Abstract

    Chromatography on controlled pore glass in combination with chaotropic buffers makes possible, in a single step, protein purifications of several hundredfold. The new emphasis is on highly selective controllable adsorption. The method is useful for the purification and concentration of ...read more

    Mentioned in this Paper

    Soluble
    Ampholytes
    Phospholipase C
    Genus staphylococcus
    Buffers
    4

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