Protein recycling and limb muscle recovery after critical illness in slow- and fast-twitch limb muscle

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Sebastien PreauMervyn Singer

Abstract

An impaired capacity of muscle to regenerate after critical illness results in long-term functional disability. We previously described in a long-term rat peritonitis model that gastrocnemius displays near-normal histology whereas soleus demonstrates a necrotizing phenotype. We thus investigated the link between the necrotizing phenotype of critical illness myopathy and proteasome activity in these two limb muscles. We studied male Wistar rats that underwent an intraperitoneal injection of the fungal cell wall constituent zymosan or n-saline as a sham-treated control. Rats (n = 74) were killed at 2, 7, and 14 days postintervention with gastrocnemius and soleus muscle removed and studied ex vivo. Zymosan-treated animals displayed an initial reduction of body weight but a persistent decrease in mass of both lower hindlimb muscles. Zymosan increased chymotrypsin- and trypsin-like proteasome activities in gastrocnemius at days 2 and 7 but in soleus at day 2 only. Activated caspases-3 and -9, polyubiquitin proteins, and 14-kDa fragments of myofibrillar actin (proteasome substrates) remained persistently increased from day 2 to day 14 in soleus but not in gastrocnemius. These results suggest that a relative proteasome deficiency in s...Continue Reading

References

Aug 1, 1991·The Journal of Pathology·T R HelliwellJ M Bone
Feb 1, 1985·Acta Physiologica Scandinavica·K Henriksson-LarsénM L Wretling
Aug 1, 1987·Brain : a Journal of Neurology·D W ZochodneW A Sibbald
Aug 1, 1986·Archives of Surgery·R J GorisP H Schillings
Jan 1, 1973·Journal of the Neurological Sciences·M A JohnsonD Appleton
Nov 1, 1984·The American Journal of Anatomy·R B Armstrong, R O Phelps
Aug 13, 1999·The American Journal of Physiology·S C HoblerP O Hasselgren
Mar 10, 2000·Biochimica Et Biophysica Acta·H R Stennicke, G S Salvesen
Mar 29, 2000·Biochemical and Biophysical Research Communications·D FischerP O Hasselgren
Dec 11, 2002·JAMA : the Journal of the American Medical Association·Bernard De JongheUNKNOWN Groupe de Réflexion et d'Etude des Neuromyopathies en Réanimation
Aug 6, 2004·The Proceedings of the Nutrition Society·Daniel TaillandierDidier Attaix
Nov 30, 2004·International Journal of Experimental Pathology·Jana KadlcíkováBenedikt M Kessler
Nov 30, 2004·Critical Care : the Official Journal of the Critical Care Forum·François KerbaulCatherine Guidon
Mar 24, 2005·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Maria KlaudeJan Wemerman
Sep 13, 2006·International Journal of Experimental Pathology·Roman SafránekMilan Holecek
Dec 7, 2007·Cell Metabolism·Cristina MammucariMarco Sandri
Jan 17, 2008·International Journal of Experimental Pathology·Tomas MuthnyMilan Holecek
Apr 21, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·G S SupinskiL A Callahan
Dec 1, 2009·Cell Metabolism·Eva MasieroMarco Sandri
Apr 8, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bin ZhengS Russ Price
Jun 12, 2010·American Journal of Respiratory and Critical Care Medicine·Jane E CarréMervyn Singer
Jan 29, 2011·The Journal of Clinical Endocrinology and Metabolism·Ilse VanhorebeekGreet Van den Berghe
Apr 8, 2011·The New England Journal of Medicine·Margaret S HerridgeUNKNOWN Canadian Critical Care Trials Group
Sep 20, 2011·Critical Care Medicine·Sarah DerdeIlse Vanhorebeek
Sep 29, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Xuejun WangSaul R Powell
Oct 4, 2011·Critical Care Medicine·Jeffrey BierbrauerSteffen Weber-Carstens
Apr 17, 2012·Cell·Mathieu Laplante, David M Sabatini
Sep 3, 2013·Biochimica Et Biophysica Acta·Marion Schmidt, Daniel Finley
Oct 11, 2013·JAMA : the Journal of the American Medical Association·Zudin A PuthuchearyHugh E Montgomery
Oct 22, 2013·Analytical Biochemistry·Ziyou CuiAldrin V Gomes
Nov 16, 2013·Cellular and Molecular Life Sciences : CMLS·Anthony M J SanchezHenri Bernardi
Apr 25, 2014·The New England Journal of Medicine·John P Kress, Jesse B Hall
Aug 7, 2014·American Journal of Physiology. Endocrinology and Metabolism·Sue C Bodine, Leslie M Baehr
Aug 15, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Anthony M J SanchezRobin B Candau
Feb 24, 2015·Critical Care Medicine·Neil E HillMervyn Singer
Jul 3, 2015·Physiological Reviews·O FriedrichL Larsson

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Citations

Sep 29, 2020·Neural Regeneration Research·Kenneth Maiese
Mar 17, 2020·EMBO Molecular Medicine·Jean-Marc CavaillonTomasz Skirecki
Jul 27, 2021·Physiological Reports·Orlando LaitanoThomas L Clanton
Aug 26, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Misa HoriikeShigeo Kawada

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Methods Mentioned

BETA
ubiquitination
lipidation
protein assay
proteasome assay
biopsy

Software Mentioned

ImageJ
GraphPad

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