Protein Reduction and Dialysis-Free Work-Up through Phosphines Immobilized on a Magnetic Support: TCEP-Functionalized Carbon-Coated Cobalt Nanoparticles

Chemistry : a European Journal
Adrian ZwyssigWendelin J Stark

Abstract

Tris(2-carboxyethyl)phosphine (TCEP) is an often-used reducing agent in biochemistry owing to its selectivity towards disulfide bonds. As TCEP causes undesired consecutive side reactions in various analytical methods (e.g., gel electrophoresis, protein labeling), it is usually removed by means of dialysis or gel filtration. Here, an alternative method of separation is presented, namely the immobilization of TCEP on magnetic nanoparticles. This magnetic reagent provides a simple and rapid approach to remove the reducing agent after successful reduction. A reduction capacity of 70 μmol per gram of particles was achieved by using surface-initiated atom transfer polymerization.

References

Jul 12, 2002·Analytical and Bioanalytical Chemistry·Christian K RienerHermann J Gruber
Sep 11, 2004·Proteome Science·Guennadi A KhoudoliJason R Swedlow
Mar 21, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Krzysztof MatyjaszewskiAndy Kusumo
Mar 2, 2010·Journal of the American Society for Mass Spectrometry·Peiran LiuReb J Russell
Jul 21, 2010·Chemistry : a European Journal·Alexander SchätzWendelin J Stark
Dec 7, 2011·Chemical Society Reviews·Yuxin LiuPeng Chen
Feb 5, 2014·BioTechniques·Lisa C Shriver-LakeChris Rowe Taitt
Mar 29, 2014·Bioconjugate Chemistry·Vladimir ZlateskiRobert N Grass
Oct 18, 2014·The Journal of Organic Chemistry·Elia M SchneiderWendelin J Stark
Dec 19, 2014·Chemical Communications : Chem Comm·C J HoferW J Stark
Apr 1, 2015·Nature Communications·Antoine MaruaniStephen Caddick
Nov 20, 2015·Chemical Communications : Chem Comm·Elia M SchneiderWendelin J Stark

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