Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve

Journal of Proteomics
Reynir SchevingIrmgard Tegeder

Abstract

Nitric oxide is a pain signaling molecule and exerts its influence through two primary pathways: by stimulation of soluble guanylylcyclase and by direct S-nitrosylation (SNO) of target proteins. We assessed in the spinal cord the SNO-proteome with two methods, two-dimensional S-nitrosothiol difference gel electrophoresis (2D SNO-DIGE) and SNO-site identification (SNOSID) at baseline and 24h after sciatic nerve injury with/without pretreatment with the nitric oxide synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME). After nerve injury, SNO-DIGE revealed 30 proteins with increased and 23 proteins with decreased S-nitrosylation. SNO-sites were identified for 17 proteins. After sham surgery only 3 proteins were up-nitrosylated. L-NAME pretreatment substantially reduced both constitutive and nerve injury evoked up-S-nitrosylation. For the top candidates S-nitrosylation was confirmed with the biotin switch technique and time course analyses at 1 and 7days showed that SNO modifications of protein disulfide isomerase, glutathione synthase and peroxiredoxin-6 had returned to baseline within 7days whereas S-nitrosylation of mitochondrial aconitase 2 was further increased. The identified SNO modified proteins are involved in mito...Continue Reading

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Citations

Oct 23, 2013·Antioxidants & Redox Signaling·Maike KanngiesserIrmgard Tegeder
Feb 25, 2014·Biochimica Et Biophysica Acta·Stefan DröseIlka Wittig
Feb 24, 2016·Frontiers in Physiology·Kelly M BalmantSixue Chen
Nov 7, 2015·Journal of Proteome Research·Zhe QuZezong Gu
Oct 13, 2015·Free Radical Biology & Medicine·Lucie ValekIrmgard Tegeder
Aug 19, 2015·Redox Biology·Katarzyna Wojdyla, Adelina Rogowska-Wrzesinska
Sep 27, 2012·International Journal of Cancer. Journal International Du Cancer·Irmgard Tegeder
Jun 19, 2013·Mass Spectrometry Reviews·Laura M López-SánchezAntonio Rodríguez-Ariza
Dec 15, 2012·Journal of Proteomics·Daymi CamejoFrancisca Sevilla
Nov 16, 2016·Trends in Neurosciences·Peter M GraceLinda R Watkins
Mar 3, 2015·Molecular BioSystems·Virginia CorreaniM Eugenia Schininà
Dec 12, 2018·Oxidative Medicine and Cellular Longevity·María Gómez-SerranoBelén Peral
Nov 5, 2019·Free Radical Biology & Medicine·Reiko Sakaguchi, Yasuo Mori
Mar 2, 2021·Neural Regeneration Research·Shuang ZhuLi-Jun Lin
Mar 31, 2021·Electrophoresis·Anna M Majewska, Agnieszka Mostek
Jul 2, 2021·Redox Biology·Bao Ngoc TranIrmgard Tegeder

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