PMID: 2496436Jan 1, 1989Paper

Protein secretion by the endometrium during pregnancy in the ewe

Reproduction, Fertility, and Development
X ZhangG M Stone

Abstract

Mature Merino ewes were surgically prepared so that after mating they became unilaterally pregnant. Ewes were killed between days 91 and 124 of pregnancy and the uterine milk was collected from the ligated uterine horn. Explants of intercaruncular and intercotyledonary endometrium were collected from the ligated and unligated uterine horns, respectively, and incubated in the presence of L-[4,5-3H]leucine to determine in vitro rates of protein synthesis and secretion. Protein:DNA and DNA:wet tissue ratios were also determined in the explants from some ewes. The proteins secreted in vitro and those present in uterine milk were partially characterized, using several procedures. Antisera raised in rabbits against uterine milk and absorbed against plasma from an ovariectomized ewe were used to detect uterine milk antigens in a variety of tissues and plasma. An average of 150 +/- 26 mL of creamy coloured uterine milk containing 30.7 +/- 4.6 mg mL-1 protein was recovered from 14 ewes. The rates of protein synthesis and secretion in vitro and the protein:DNA ratio were all higher in intercotyledonary than in intercaruncular endometrium, but the predominant radiolabelled protein(s) secreted in vitro by both types of endometrium was show...Continue Reading

Citations

Nov 5, 1997·Analytical Biochemistry·J LippincottI Apostol

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