PMID: 2502537Aug 1, 1989Paper

Protein secretion in Pseudomonas aeruginosa: molecular cloning and characterization of the xcp-1 gene

Journal of Bacteriology
M BallyA Lazdunski

Abstract

Pleiotropic mutations (xcp) affecting the secretion of proteins in Pseudomonas aeruginosa have been previously characterized and mapped at three different loci on the chromosome. The xcp-1 gene, which is located at the 0-min region, was isolated from a genomic bank containing DNA from P. aeruginosa PAO1. The recombinant cosmid pLX25 complemented the xcp::Tn5-751 insertion mutation previously described. The xcp-1 gene was located on pLX25 by mapping the insertion point of transposon Tn5-751 and by deletion and subcloning analysis. The xcp-1 gene was expressed when transcription was initiated from a tac promoter, and a 26,000-dalton protein was identified in Escherichia coli minicells. The Xcp-1 protein was associated with the membrane fraction of E. coli. A 30-kilodalton outer membrane protein was also affected by the xcp::Tn5-751 mutation in P. aeruginosa. The possible correlation between Xcp-1 and this protein is discussed.

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Citations

Feb 6, 1998·FEMS Microbiology Reviews·E S Stanislavsky, J S Lam
Jul 1, 1990·Molecular Microbiology·R E HancockN Martin
Jul 2, 1999·Applied and Environmental Microbiology·S IuresciaE Zennaro
Mar 18, 2000·Infection and Immunity·V AragonN P Cianciotto
Dec 19, 2000·Infection and Immunity·V AragonN P Cianciotto
Feb 1, 1990·Biochimie·A LazdunskiM Murgier
Dec 1, 1996·Applied and Environmental Microbiology·C LabarreJ Guzzo
Jun 1, 1997·Applied and Environmental Microbiology·F BeltramettiE Zennaro
Jan 1, 1996·Applied and Environmental Microbiology·A M MarconiE Zennaro

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