PMID: 8593182Aug 1, 1995Paper

Protein selectivity in immobilized metal affinity chromatography based on the surface accessibility of aspartic and glutamic acid residues

Journal of Protein Chemistry
M Zachariou, M T Hearn

Abstract

The interaction of different species variants of cytochrome c and myoglobin, as well as hen egg white lysozyme, with the hard Lewis metal ions Al3+, Ca2+, Fe3+, and Yb3+ and the borderline metal ion Cu2+, immobilized to iminodiacetic acid (IDA)-Sepharose CL-4B, has been investigated over the range pH 5.5-8.0. With appropriately chosen buffer and metal ion conditions, these proteins can be bound to the immobilized Mn+-IDA adsorbents via negatively charged amino acid residues accessible on the protein surface. For example, tuna heart cytochrome c, which lacks surface-accessible histidine residues, readily bound to the Fe3+-IDA adsorbent, while the other proteins also showed affinity toward immobilized Fe3+-IDA adsorbents when buffers containing 30 mM of imidazole were used. These studies document that protein selectivity can be achieved with hard-metal-ion immobilized metal ion affinity chromatography (IMAC) systems through the interaction of surface-exposed aspartic and glutamic acid residues on the protein with the immobilized Mn+-IDA complex. These investigations have also documented that the so-called soft or borderline immobilized metal ions such as the Cu2+-IDA adsorbent can also interact with surface-accessible aspartic an...Continue Reading

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Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·V Gaberc-Porekar, V Menart
Jan 9, 2015·Preparative Biochemistry & Biotechnology·Ladawan Nakchum, Sang Moo Kim
Sep 13, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Khadija El IdrissiChristian Rolando
Jun 16, 2017·Nutrients·Yanan LiGuangrong Huang
Oct 10, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Ömür AcetMehmet Odabaşı
Mar 30, 2021·Applied Biochemistry and Biotechnology·Ömür AcetMehmet Odabaşı
May 19, 2009·Journal of Agricultural and Food Chemistry·Ying LvShuntang Guo
Mar 22, 2003·Journal of Chromatography. a·E K M UedaL Morganti

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