Protein self-assembly and lipid binding in the folding of the potassium channel KcsA

Biochemistry
Francisco N BarreraJose M González-Ros

Abstract

Moderate concentrations of the alcohol 2,2,2-trifluoroethanol (TFE) cause the coupled unfolding and dissociation into subunits of the homotetrameric potassium channel KcsA, in a process that is partially irreversible when the protein is solubilized in plain dodecyl beta-d-maltoside (DDM) micelles [Barrera et al. (2005) Biochemistry 44, 14344-52]. Here we report that the transition from the folded tetramer to the unfolded monomer becomes completely reversible when KcsA is solubilized in mixed micelles composed of the detergent DDM and the lipids DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and DOPG (1,2-dioleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]). This result suggests that lipids may act as effectors in the tetramerization of KcsA. The observed reversibility allowed the determination of the standard free energy of the folding reaction of KcsA: DeltaG = 30.5 +/- 3.1 kcal x mol-1. We also observed that, prior to the unfolding of the tetramer, the presence of lower TFE concentrations causes the disassembly of supramolecular clusters of KcsA into the individual tetrameric molecules. Within the limits of experimental resolution, this is also a reversible process, but unlike the tetramer to monomer transition from above,...Continue Reading

References

Nov 20, 1993·Journal of Molecular Biology·S NussbergerW Kühlbrandt
Nov 25, 2000·Biochimica Et Biophysica Acta·D LichtenbergM M Kozlov
Jun 25, 2002·Journal of Molecular Biology·Tara Nath NiraulaKazuyuki Akasaka
Aug 7, 2002·FEBS Letters·Annemieke van DalenBen de Kruijff
Apr 23, 2003·FEBS Letters·Jeroen A A DemmersJ Antoinette Killian
May 4, 2006·The Journal of Biological Chemistry·Maria L MolinaJose M González-Ros
Jun 22, 2006·Journal of Molecular Biology·Anna L Mallam, Sophie E Jackson

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Citations

Nov 1, 2011·Biochemistry·Anbazhagan VeerappanDirk Schneider
Nov 3, 2011·Biochemistry·Prasanna K DevaraneniFrancis I Valiyaveetil
Jul 17, 2012·Nature Chemical Biology·Rosanna P Baker, Sinisa Urban
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gerard H M HuysmansSheena E Radford
Feb 12, 2011·The Journal of Biological Chemistry·Manuela GebhardtGerhard Thiel
Dec 3, 2014·Biochimica Et Biophysica Acta·Noreen KleinDirk Schneider
Jul 19, 2011·Current Opinion in Structural Biology·Alessandro Senes
Jul 25, 2014·Archives of Biochemistry and Biophysics·Jennifer NeumannDirk Schneider
Feb 18, 2014·Journal of Molecular Biology·Nicola J HarrisPaula J Booth
Nov 20, 2012·Biochimica Et Biophysica Acta·Zakhar O ShenkarevAlexander S Arseniev
Nov 12, 2013·Biochimica Et Biophysica Acta·J A PovedaJ M González-Ros
Jan 23, 2009·Current Opinion in Structural Biology·Paula J Booth, Paul Curnow
Aug 25, 2009·Journal of Molecular Biology·David MillerPaula J Booth
Nov 22, 2011·Biochimica Et Biophysica Acta·Nicola J Harris, Paula J Booth
Nov 26, 2014·Quarterly Reviews of Biophysics·Jonathan P Schlebach, Charles R Sanders
Dec 3, 2014·Archives of Biochemistry and Biophysics·John C DeaconFrancisco N Barrera
Mar 18, 2016·PloS One·Natalie Di BartoloPaula J Booth
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·M R SandersP J Booth
Feb 13, 2008·Journal of Cellular and Molecular Medicine·Pablo V EscribáGwendolyn Barceló-Coblijn
May 24, 2011·Biochemical Society Transactions·Natalie DiBartolo, Paula J Booth
Aug 2, 2017·Scientific Reports·Masayuki Iwamoto, Shigetoshi Oiki
Apr 11, 2020·International Journal of Molecular Sciences·María Lourdes RenartJosé Antonio Poveda
Nov 11, 2016·Nanotechnology·David M MillerPaula J Booth
Oct 16, 2020·Protein Science : a Publication of the Protein Society·Karolina Corin, James U Bowie
Jul 28, 2019·Biochimica Et Biophysica Acta. Biomembranes·José A PovedaCarmen Domene
Oct 12, 2010·Biochemistry·M L RenartJ M González Ros
Aug 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kevin C SongTobin R Sosnick
Jul 17, 2020·Biochimica Et Biophysica Acta. Biomembranes·Victoria OakesCarmen Domene

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