Protein stability in ice

Biophysical Journal
G B Strambini, Margherita Gonnelli

Abstract

This study presents an experimental approach, based on the change of Trp fluorescence between native and denatured states of proteins, which permits to monitor unfolding equilibria and the thermodynamic stability (DeltaG degrees ) of these macromolecules in frozen aqueous solutions. The results obtained by guanidinium chloride denaturation of the azurin mutant C112S from Pseudomonas aeruginosa, in the temperature range from -8 to -16 degrees C, demonstrate that the stability of the native fold may be significantly perturbed in ice depending mainly on the size of the liquid water pool (V(L)) in equilibrium with the solid phase. The data establish a threshold, around V(L)=1.5%, below which in ice DeltaG degrees decreases progressively relative to liquid state, up to 3 kcal/mole for V(L)=0.285%. The sharp dependence of DeltaG degrees on V(L) is consistent with a mechanism based on adsorption of the protein to the ice surface. The reduction in DeltaG degrees is accompanied by a corresponding decrease in m-value indicating that protein-ice interactions increase the solvent accessible surface area of the native fold or reduce that of the denatured state, or both. The method opens the possibility for examining in a more quantitative f...Continue Reading

References

Oct 1, 1995·Protein Science : a Publication of the Protein Society·J K MyersJ M Scholtz
Feb 1, 1996·Biophysical Journal·G B Strambini, E Gabellieri
Dec 1, 1996·Journal of Pharmaceutical Sciences·B S ChangJ F Carpenter
Oct 3, 1999·European Journal of Biochemistry·G MeiA Finazzi-Agrò
Mar 24, 2004·Nature Reviews. Microbiology·Georges Feller, Charles Gerday
Sep 25, 2004·Protein Science : a Publication of the Protein Society·Anders SandbergB Göran Karlsson
Feb 8, 2006·Biophysical Journal·Edi Gabellieri, Giovanni B Strambini
Apr 6, 2006·EMBO Reports·Salvino D'AmicoCharles Gerday

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Citations

Jun 8, 2013·The Journal of Physical Chemistry. B·Alan TwomeyAlptekin Aksan
Sep 1, 2011·Biopreservation and Biobanking·Allison HubelXiao Zhong
Feb 13, 2013·Journal of Pharmaceutical Sciences·Maria A MillerJennifer A Maynard
May 7, 2014·Journal of Pharmaceutical Sciences·Koreyoshi ImamuraKazuhiro Nakanishi
May 17, 2014·Journal of Pharmaceutical Sciences·Lu LiuJohn F Carpenter
Jan 24, 2014·Journal of Pharmaceutical Sciences·Srivalli N TelikepalliDavid B Volkin
Oct 1, 2008·Journal of Pharmaceutical Sciences·Hanns-Christian MahlerSylvia Kiese
Sep 24, 2015·Journal of Pharmaceutical Sciences·Brian D ConnollyPhilippe Lam
Sep 3, 2011·Biophysical Chemistry·Edi GabellieriPatrizia Cioni
Dec 1, 2015·International Journal of Biological Macromolecules·Sumit K ChaturvediRizwan H Khan
Dec 7, 2007·Biophysical Journal·Vishard Ragoonanan, Alptekin Aksan
Apr 19, 2015·International Journal of Pharmaceutics·Alan TwomeyAlptekin Aksan
Aug 10, 2010·Biochimica Et Biophysica Acta·Iona P Garber CohenF Luis González Flecha
Mar 28, 2012·The Journal of Physical Chemistry. B·Shin G ChouEvgenyi Y Shalaev
Jan 10, 2017·Bioscience, Biotechnology, and Biochemistry·Takanori ShimizuKoreyoshi Imamura
May 10, 2016·International Journal of Biological Macromolecules·Kinjal A PatelIpsita Roy
Feb 2, 2018·Applied Microbiology and Biotechnology·Arshad Ahmed PadhiarMintao Zhong
Mar 18, 2020·European Journal of Ophthalmology·Eduardo AnituaJesús Merayo-Lloves
Jul 21, 2020·Biotechnology and Bioengineering·Melissa HolsteinZheng Jian Li
Oct 27, 2007·Pharmaceutical Development and Technology·Bakul S BhatnagarMichael J Pikal
Dec 29, 2020·Journal of Pharmaceutical Sciences·William J RayfieldSunitha Kandula
Apr 3, 2020·Journal of Pharmaceutical Sciences·Andrea Arsiccio, Roberto Pisano
Jun 22, 2019·International Journal of Biological Macromolecules·Celeste RodriguezXin Wen
Jul 31, 2008·The Journal of Physical Chemistry. B·Giovanni B Strambini, Margherita Gonnelli
Apr 11, 2009·The Journal of Physical Chemistry. B·Dushyant B VarshneyEvgenyi Y Shalaev
Feb 26, 2008·Biochemistry·Giovanni B Strambini, Margherita Gonnelli
Jul 28, 2011·The Journal of Physical Chemistry. B·Ryan D BookerSatish K Singh
Mar 4, 2020·Journal of the American Chemical Society·Andrea ArsiccioJoan-Emma Shea

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