Protein stability influences human immunodeficiency virus type 2 Vpr virion incorporation and cell cycle effect

Virology
V N KewalramaniM Emerman

Abstract

Using recombinant proteins as standards, we calculated the amount of Vpr and Vpx present in HIV-2ROD particles. We find 2000-3000 copies of Vpx per particle but only 40-50 copies of Vpr. We investigated the reasons for this discrepancy between Vpx and Vpr and found that viral factors, including HIV-2 Vpx, do not restrict its incorporation. Instead, the accumulation of HIV-2ROD Vpr during infection is restricted by a short protein half-life which acts to limit its virion incorporation. The half-life of HIV-2 Vpr was calculated to be about 90 min, while HIV-2 Vpx and HIV-1 Vpr had half-lifes of 36 and 20 hr, respectively. Moreover, while both HIV-1 Vpr and HIV-2 Vpr cause cells to accumulate in G2 of the cell cycle, the effect of HIV-2 Vpr is attenuated relative to HIV-1 Vpr. Thus, protein stability correlates with both the function of Vpr and its virion incorporation.

Citations

Jan 4, 2013·Journal of Proteome Research·Cameron J SchweitzerMichael Belshan
Sep 23, 2000·Journal of Virology·C A PatelR J Pomerantz
Oct 28, 2004·Journal of Virology·Jörg G BaumannVineet N KewalRamani
Apr 13, 2010·Retrovirology·Diana AyindeFlorence Margottin-Goguet
Feb 1, 2012·PloS One·Xiaoyun WenCarlos M C de Noronha
Nov 26, 2009·Uirusu·Masako Nomaguchi, Akio Adachi
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A StewartI S Chen
Jan 1, 1997·Springer Seminars in Immunopathology·C Jassoy, B D Walker
Nov 19, 2003·Biochemical and Biophysical Research Communications·Mari MatsudaMichiaki Masuda
Jan 2, 2014·Protein Expression and Purification·Karen HänelAngelika Loidl-Stahlhofen
Sep 1, 1996·Current Biology : CB·M Emerman
Jun 7, 2016·Reviews in Medical Virology·Rui SoaresTeresa Gonçalves
Jul 12, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Karuppiah MuthumaniDavid B Weiner
Apr 11, 2014·Journal of Virology·Hamayun John SharifiCarlos M C de Noronha
Oct 28, 2019·Cells·Helena Fabryova, Klaus Strebel
Jul 1, 1997·Molecular and Cellular Biology·J GuS Sandmeyer

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