Protein subcellular localization profiling of breast cancer cells by dissociable antibody microarray staining.

Proteomics
Guanyuan FuG Wayne Zhou

Abstract

We have developed dissociable antibody microarray (DAMA) staining technology that provides a new approach to the global analysis of protein subcellular localization (SCL) in fixed cells. We have developed and optimized this technology for protein SCL profiling, generated ChipView, a program for management and analysis of molecular image database, and utilized the technique to identify proteins with unique SCL in breast cancer cell lines. We compared the SCL profiles of 325 proteins among nine different breast cell lines, and have identified one protein, Cyclin B1, with distinctively different SCLs between normal and cancer cell lines. With classic individual immunostaining, Cyclin B1 was confirmed to localize to the cytoplasm of seven breast cancer cell lines and in both cytoplasm and nuclei of two normal breast cell lines, and to have higher expression levels in the cancer cell lines tested.

References

Feb 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W A StainesT Hökfelt
Jan 1, 1994·Annual Review of Cell Biology·U SiebenlistK Brown
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Apr 5, 2000·Nature Biotechnology·A Q Emili, G Cagney
Apr 12, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·D Q DingY Hiraoka
Sep 6, 2000·Nature Biotechnology·R M de WildtI M Tomlinson
Mar 17, 2001·Chemistry & Biology·T Kodadek
Nov 27, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Z E WintersC J Norbury
Nov 28, 2001·Proteomics·V KnezevicD B Krizman
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·X MorinW Chia
Jan 10, 2002·Cell Biology International·Katherine L B BarrettKaren S Katula
Mar 5, 2002·Nature Medicine·William H RobinsonPaul J Utz
Mar 30, 2002·Nature Biotechnology·Barry SchweitzerStephen F Kingsmore
Nov 28, 2002·Nature Genetics·Gavin MacBeath
Feb 13, 2003·European Journal of Biochemistry·Mathias Dreger
Mar 14, 2003·The Journal of Pathology·Heidi KühlingPierre Rudolph
Mar 21, 2003·Journal of Proteome Research·Ronald L WoodburyRichard C Zangar
May 13, 2003·International Journal of Cancer. Journal International Du Cancer·Pierre RudolphReza Parwaresch
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Jan 20, 2004·Proteomics·Yingjian Wang
Mar 23, 2004·Journal of Anatomy·Matthias SchiekerStefan Milz
Jun 14, 2005·Expert Opinion on Therapeutic Targets·Ruo-Pan HuangRuochun Huang
Aug 30, 2005·Molecular & Cellular Proteomics : MCP·Mathias UhlénFredrik Pontén
Nov 3, 2005·The FEBS Journal·Paul Bertone, Michael Snyder
Aug 24, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Denise J L Tan, Alfonso Martinez Arias
Nov 28, 2006·Mechanisms of Ageing and Development·David A HallMichael Snyder
Mar 16, 2007·Cancer Science·Takashi SuzukiHironobu Sasano
Mar 23, 2007·Biomolecular Engineering·Ronny FalkSophia Hober
Oct 16, 2007·Molecular & Cellular Proteomics : MCP·X Cynthia SongG Wayne Zhou
Nov 8, 2007·Nucleic Acids Research·Josefine SprengerRohan D Teasdale
Aug 2, 2008·Molecular & Cellular Proteomics : MCP·Lisa BerglundMathias Uhlén

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Citations

Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga

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