PMID: 6170733Nov 1, 1981Paper

Protein synthesis and axonal transport during nerve regeneration

Journal of Neurochemistry
G W Perry, D L Wilson

Abstract

Protein synthesis and axonal transport have been studied in regenerating peripheral nerves. Sciatic nerves of bullfrogs were unilaterally crushed or cut. The animals were killed 1, 2, or 4 weeks later, and 8th and 9th dorsal root ganglia removed together with sciatic nerves and dorsal roots. The ganglia were selectively labeled in vitro with [35S]-methionine. Labeled proteins, in dorsal root ganglia and rapidly transported to ligatures placed on the sciatic nerves and dorsal roots, were analyzed by two-dimensional polyacrylamide gel electrophoresis. Qualitative analysis of protein patterns revealed no totally new proteins synthesized or rapidly transported in regenerating nerves. However, quantitative comparison of regenerating and contralateral control nerves revealed significant differences in abundance for some of the proteins synthesized in dorsal root ganglia, and for a few of the rapidly transported proteins. Quantitative analysis of rapidly transported proteins in both the peripheral processes (spinal nerves) and central processes (dorsal roots) revealed similar changes despite the fact that the roots were undamaged. The overall lack of drastic changes seen in protein synthesis and transport suggests that the neuron in i...Continue Reading

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Citations

Jun 13, 1983·Brain Research·D L Edwards, B Grafstein
Nov 1, 1988·Journal of the Neurological Sciences·R Pamphlett
Sep 1, 1989·Journal of the Neurological Sciences·R Pamphlett
Apr 1, 1990·Journal of Neuroscience Methods·M EdbladhA Edström
Sep 18, 1992·Brain Research. Developmental Brain Research·B Tedeschi, F J Liuzzi
Jan 1, 1992·Electron Microscopy Reviews·K Meller
Mar 1, 1986·The Journal of Hand Surgery·A L OstermanJ R Urbaniak
Jan 1, 1987·CRC Critical Reviews in Biochemistry·M Schwartz
Nov 1, 1990·Journal of Neurochemistry·A BurianiD J Goldberg
Mar 1, 1990·Journal of Neurobiology·J A Menéndez, S C Cubas
May 1, 2009·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Ilona Klusáková, Petr Dubový
Dec 16, 1982·Brain Research·R J Wenthold, M L McGarvey
Jun 1, 1983·Journal of Neurochemistry·G W PerryD L Wilson
Dec 1, 1987·Journal of Neurochemistry·D C Larrivee, B Grafstein
Dec 24, 2013·Seminars in Cell & Developmental Biology·Carolina González, Andrés Couve
May 1, 1988·Muscle & Nerve·R S Pamphlett

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