PMID: 6795May 1, 1976

Protein synthesis by a cell-free preparation from the bird malaria, Plasmodium lophurae

The Journal of protozoology
I W Sherman, L A Jones

Abstract

Cytoplasmic polyribosomes were isolated from the avian malaria parasite Plasmodium lophurae by lysis with 0.15% Triton X-100 followed by high speed centrifugation through a discontinuous sucrose gradient. Polyribosomes were protected from nuclease degradation using 100 mug/ml heparin or 50 mug/ml dextran sulfate. Cell-free incorporation of radioisotope-labeled amino acids required a pH 5 fraction (duck reticulocyte), Mg2+, and an energy-generating system. The protein synthesizing system was stimulated by the addition of polyuridylic acid. Optimum conditions for protein synthesis by the plasmodial system are described. The effects of drugs on the cell-free protein synthesizing system using duck reticulocyte and plasmodial ribosomes are reported.

References

Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·I W Sherman
Nov 1, 1975·The Journal of protozoology·I W ShermanJ Williamson
Dec 29, 1972·Journal of Molecular Evolution·P A Lava-SanchezA L Posta
Aug 24, 1973·Biochimica Et Biophysica Acta·L Carrasco, D Vazquez
Apr 15, 1970·Biochimica Et Biophysica Acta·R Ascione, R B Arlinghaus
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Citations

Dec 1, 1982·Molecular and Biochemical Parasitology·M Wallach
Feb 1, 1983·Molecular and Biochemical Parasitology·J F da Silveira, O Mercereau-Puijalon
May 1, 1977·The Journal of protozoology·I W Sherman, L A Jones
Sep 1, 1982·Bioscience Reports·C A Gritzmacher, R T Reese
Apr 1, 1984·Annals of Tropical Medicine and Parasitology·A YayonH Ginsburg

Related Concepts

Cell-Free System
Ducks
Hydrogen-Ion Concentration
Magnesium
Endorphenyl
Plasmodium
Poly U
Polyribosomes
Reticulocytes
Protein Biosynthesis

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