PMID: 1206797Jan 1, 1975Paper

Protein synthesis in BHK-21 cells infected with semliki forest virus

Journal of Virology
G Wengler

Abstract

[3H]leucine-labeled proteins synthesized in BHK-21 cells infected with Semliki Forest virus were fractionated by polyacrylamide gel electrophoresis (PAGE). Cellular and virus-specific proteins were identified by difference analysis of the PAGE profiles. The specific activity of intracellular [3H-A1leucine was determined. Two alterations of protein synthesis, which develop with different time courses, were discerned. (i) In infected cultures an inhibition of overall protein synthesis to about 25% of the protein synthesis in mock-infected cultures develops between about 1 and 4 h postinfection (p.i.). (ii) The relative amount of virus-specific polypeptides versus cellular polypeptides increases after infection. About 80% of the proteins synthesized at 4 h p.i. are cellular proteins. Since significant amounts of nontranslocating robosomes in polyribosomes were not detected up to 7 h p.i., the inhibition of protein synthesis is not caused by inactivation of about 75% of all polyribosomes but by a decreased protein synthetic activity of the majority of polyribosomes. Indirect evidence indicates that an inhibition of elongation and/or release of protein synthesis develops in infected cells, which is sufficient to account for the obse...Continue Reading

References

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Citations

Feb 9, 2000·Journal of Virology·S I IsmailM Uden
Jun 8, 2007·The Journal of General Virology·Stefan A Deuber, Jovan Pavlovic
Jan 1, 1980·Pharmacology & Therapeutics·L Carrasco, A E Smith

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