PMID: 18631655Jun 1, 1980

Protein synthesis in cells infected by Autographa californica nuclear polyhedrosis virus (Ac-NPV): the effect of cytosine arabinoside

Virology
P Dobos, M A Cochran

Abstract

Twenty-two virion polypeptides (VP) were detected reproducibly when the occluded form (OV) of [35S]methionine-labeled Ac-NPV, purified from polyhedral inclusion bodies (PIB), was analyzed by polyacrylamide gel electrophoresis and autoradiography. Ten of the VPs and the polyhedral protein (PP) were phosphorylated and 6 VPs were glycoproteins. Purified, nonoccluded virus (NOV) revealed 25 polypeptides. During a single cycle of virus replication, the synthesis of 33 infected cell polypeptides (1CP) was detected in different relative proportions at different times. They were arbitrarily designated as early (0-12hr), middle (12-18 hr), and late 18-24 hr) polypeptides. Most of the middle and late proteins seemed to be viral structural proteins. Rapid post-translational cleavage of IPCs was not observed; however, pulse-chase experiments revealed post-translational modifications of at least four polypeptides. Inhibition of DNA synthesis at the time of infection did not prevent the synthesis of ICPs, VPs, or the formation of PIBs. Progeny PIB from both control and cytosine arabinoside (Ara C)-treated cells that were infected with radiochemically pure [3H]thymidine-labeled NOV contained parental virus DNA. Electron micrographs of thin se...Continue Reading

References

Jan 1, 1975·Intervirology·E M DoughertyC F Reichelderfer
Jun 1, 1977·Virology·K A HarrapJ S Robertson
Feb 1, 1978·Virology·M D Summers, G E Smith
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D F Summers, J V Maizel
Jan 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J HudsonR Weil
Dec 1, 1974·Journal of Ultrastructure Research·E A MacKinnonP Faulkner
Aug 14, 1967·Journal of Molecular Biology·K I Oda, W K Joklik
Sep 1, 1978·Virology·G E Smith, M D Summers

Citations

Jan 1, 1996·Cytotechnology·G V Williams, P Faulkner
Oct 1, 1986·Virus Research·T H MainprizeL K Miller
Aug 15, 1992·European Journal of Biochemistry·T GärtnerH Rübsamen-Waigmann
Jan 1, 1990·Annals of the New York Academy of Sciences·M L ShulerH A Wood
Oct 1, 1988·Virology·B G Ooi, L K Miller
Sep 10, 2003·International Reviews of Immunology·Thomas E Clarke, Rollie J Clem

Related Concepts

Autoradiography
Cytarabine
DNA, Viral
Polyacrylamide Gel Electrophoresis
Glycoproteins
Nucleopolyhedrovirus
Phosphorylation
Post-Translational Protein Processing
Thymidine
Viral Structural Proteins

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