Protein synthesis in lactating guinea-pig mammary tissue perfused in vitro. I. Radiolabelling of membrane and secretory proteins

Experimental Cell Research
I H MatherT B Mepham

Abstract

A method for the in vitro perfusion of isolated guinea-pig mammary tissue is described that allows the radiolabelling of secretory and membrane proteins. Glands were depleted of methionine, labelled with [35S]methionine for 5 min and perfused with medium containing an excess of unlabelled methionine for varying times. The structural integrity of the alveoli in the perfused glands appeared well maintained. Epithelial polarity was preserved and junctional complexes were evident. About 20% of the methionine provided in the medium was extracted by glands of 10 g wet weight under the labelling conditions employed. With chase periods from 15 to 40 min, 50-70% of the methionine was incorporated into trichloroacetic-acid (TCA)-precipitable material. The principal radiolabelled proteins recovered from the tissue fractions had Mrs and isoelectric points similar to the major secretory proteins (i.e. caseins and alpha-lactalbumin) of guinea-pig milk. Autoradiography of tissue sections at the resolution of the light microscope showed that secretory proteins were transported from sites of synthesis within secretory cells to the alveolar lumina after 45 min. These highly labelled secretory proteins could be almost completely removed from micr...Continue Reading

References

Aug 1, 1978·The Biochemical Journal·R K CraigR L Hall
Oct 31, 1975·Biochimica Et Biophysica Acta·S Patton, T W Keenan
Apr 1, 1976·The Journal of Cell Biology·W W FrankeT W Keenan
Jun 1, 1973·Comparative Biochemistry and Physiology. A, Comparative Physiology·T B Mepham, N F Beck
Oct 15, 1973·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R W Fuller, B W Roush
Apr 1, 1974·Quarterly Journal of Experimental Physiology and Cognate Medical Sciences·S R Davis, T B Mepham
Mar 1, 1982·The Journal of Cell Biology·K E Howell, G E Palade
Jan 1, 1983·Differentiation; Research in Biological Diversity·G BruderI H Mather
Apr 16, 1982·Molecular and Cellular Biochemistry·C H SullivanP J Madara
Dec 1, 1981·The Journal of Cell Biology·M G Farquhar, G E Palade

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Citations

Jun 1, 1996·The Histochemical Journal·R J Van den Munckhof
Aug 13, 2013·Journal of Agricultural and Food Chemistry·Haotian ZhengDavid W Everett
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J D StubbsG Parry
Nov 8, 2012·Bioscience, Biotechnology, and Biochemistry·Hajime NakataniTsukasa Matsuda
Jun 1, 1986·Experimental Cell Research·G Bruder, B Wiedenmann

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