PMID: 6401830Jan 1, 1983Paper

Protein synthesis rates in rat muscle and skin based on Lysyl-tRNA radioactivity

The Journal of Surgical Research
T ChikenjiJ M Kinney

Abstract

Measurement of protein synthesis in individual organs is important in understanding metabolic changes in injury, sepsis, or starvation. Methods, mostly isotopic, for measuring synthesis are plagued by problems of experimental design and interpretation. Thus it is desirable to use a variety of methods based on different assumptions. The present study is the first to isolate radioactive aminoacyl-tRNA in the study of protein synthesis in muscle and skin. Male rats, 200-300 g, trained to eat chow for 4 hr/day were studied at 2 hr (absorptive) or 16 hr (postabsorptive) after a meal. Under ether anesthesia, a tracer dose of L-[4-5-3H(N)]-lysine was infused at a constant rate. At 20, 30, or 40 min 1 ml of arterial blood was withdrawn and 2-g samples of skin and thigh muscle were quickly excised and frozen. Samples were pooled from 4 to 7 rats for each infusion period. Concentrations and specific activities were determined for plasma lysine, and for free, tRNA, and protein-bound lysine in muscle and skin. Protein renewal rates in absorptive and postabsorptive periods averaged 6 and 9% per day in muscle, and 20 and 35% in skin. The data for muscle confirms results of other methods and suggests little contribution of rapidly turning ove...Continue Reading

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Citations

Oct 1, 1994·Amino Acids·D M BarnesK C Klasing
Nov 1, 1983·The Journal of Surgical Research·B McCallisterN N Abumrad
Sep 1, 1990·Baillière's Clinical Endocrinology and Metabolism·K Smith, M J Rennie
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P W WattM J Rennie
Feb 22, 2007·Annual Review of Nutrition·Noboru Mizushima, Daniel J Klionsky
Oct 1, 1988·The Journal of Surgical Research·P O HasselgrenJ E Fischer
Mar 1, 1986·The Journal of Surgical Research·P D GreigJ M Kinney
Mar 13, 2010·Seminars in Cell & Developmental Biology·Akiko Kuma, Noboru Mizushima
Aug 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R E KeenM E Phelps
Oct 26, 2005·Cell Death and Differentiation·N Mizushima
Mar 20, 1999·The Journal of Nutrition·H A JohnsonC C Calvert
Mar 1, 1994·The American Journal of Physiology·M J RennieP W Watt

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