Protein targeting and integration signal for the chloroplastic outer envelope membrane

The Plant Cell
H M Li, L J Chen

Abstract

Most proteins in chloroplasts are encoded by the nuclear genome and synthesized in the cytosol. With the exception of most quter envelope membrane proteins, nuclear-encoded chloroplastic proteins are synthesized with N-terminal extensions that contain the chloroplast targeting information of these proteins. Most outer membrane proteins, however, are synthesized without extensions in the cytosol. Therefore, it is not clear where the chloroplastic outer membrane targeting information resides within these polypeptides. We have analyzed a chloroplastic outer membrane protein, OEP14 (outer envelope membrane protein of 14 kD, previously named OM14), and localized its outer membrane targeting and integration signal to the first 30 amino acids of the protein. This signal consists of a positively charged N-terminal portion followed by a hydrophobic core, bearing resemblance to the signal peptides of proteins targeted to the endoplasmic reticulum. However, a chimeric protein containing this signal fused to a passenger protein did not integrate into the endoplasmic reticulum membrane. Furthermore, membrane topology analysis indicated that the signal inserts into the chloroplastic outer membrane in an orientation opposite to that predicted...Continue Reading

References

Apr 22, 1991·FEBS Letters·Y GavelG von Heijne
Jul 13, 1990·Cell·T SöllnerW Neupert
Dec 1, 1990·The Journal of Cell Biology·H F StegerN Pfanner
May 1, 1990·The Journal of Membrane Biology·G von Heijne
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M SalomonJ Soll
Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E HartmannH F Lodish
Jul 1, 1988·European Journal of Biochemistry·G von Heijne, Y Gavel
Mar 1, 1995·The Plant Journal : for Cell and Molecular Biology·M SeedorfJ Soll

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Citations

Feb 5, 2008·Nature Cell Biology·Jocelyn Bédard, Paul Jarvis
Nov 10, 1998·The Plant Journal : for Cell and Molecular Biology·L J Chen, H M Li
Jan 25, 2005·Plant Biology·T BeckerE Schleiff
Nov 15, 2003·The Plant Cell·Kazusato OikawaMasamitsu Wada
Apr 24, 1999·The Plant Cell·K Keegstra, K Cline
Nov 3, 2012·PLoS Biology·Yi-Shan TengHsou-min Li
Apr 28, 2007·The Plant Journal : for Cell and Molecular Biology·Elisabeth Truernit, Julian M Hibberd
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology·Nancy R Hofmann, Steven M Theg
May 10, 2008·The Plant Journal : for Cell and Molecular Biology·Nicolas FourrierGlenn Thorlby
Jun 23, 2011·Chembiochem : a European Journal of Chemical Biology·Bettina Bölter, Jürgen Soll
Aug 9, 2005·Trends in Plant Science·Nancy R Hofmann, Steven M Theg
Dec 26, 2001·Biochimica Et Biophysica Acta·E Schleiff, R B Klösgen
Jun 27, 2014·Frontiers in Plant Science·Lynn G L RichardsonDanny J Schnell
Aug 24, 1999·The Journal of Biological Chemistry·A KouranovD J Schnell
Oct 15, 2009·Plant Signaling & Behavior·John StangaPatrick H Masson
Oct 7, 2018·Photosynthesis Research·Philip M Day, Steven M Theg

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