PMID: 11710128Nov 17, 2001Paper

Protein thioacylation. 1. Reagents design and synthesis

Biomacromolecules
G LevesqueT N Pham

Abstract

Thioacylation is a new way for protein chemical modification. Carboxylic dithioesters and -acids react selectively and rapidly at room temperature with aliphatic amines such as lysine epsilon-amino groups leading to thioamide formation, without any other reagent or catalyst. Various thioacylating reagents were synthesized: monofunctional dithioesters bearing on the acylating end various chemical groups such as: aliphatic chains, phenyl group, mono- and dicarboxylic acids, dialkylphosphonic ester, phosphonic acid, thiol, phenol, or quaternary ammonium group. Bifunctional dithioesters containing either a polymethylene chain or an ethylene oxide oligomer as spacer group as well as some mono- and bis(dithio acids) are described. Applications of thioacylation may be involved either in enzyme chemical modifications or in the obtention of new materials from proteins. Bifunctional reagents might be used as cross-linking or coupling reagents.

References

Nov 27, 1979·Biochemistry·K F GeogheganR E Feeney
Jul 22, 1975·Biochemical and Biophysical Research Communications·A AzziC Richter
Jan 1, 1977·Annual Review of Biochemistry·K Peters, F M Richards
Jan 1, 1979·International Journal of Peptide and Protein Research·K FretheimR E Feeney
Mar 1, 1975·Analytical Biochemistry·S L Snyder, P Z Sobocinski
Jan 1, 1976·Journal of Pharmaceutical Sciences·N H Leon
Jan 1, 1990·Bioconjugate Chemistry·G E Means, R E Feeney
Sep 1, 1971·The Biochemical Journal·R Fields
Jun 1, 1968·Biochemistry·G E Means, R E Feeney
Mar 4, 1982·Biochimica Et Biophysica Acta·M Hollecker, T E Creighton
Sep 15, 1980·Analytical Biochemistry·O Ohara, S Takahashi
May 15, 1983·International Journal of Cancer. Journal International Du Cancer·M C GarnettR W Baldwin
Jan 1, 1983·Methods in Enzymology·T H Ji
Aug 20, 1988·Biotechnology and Bioengineering·K Ampon, G E Means
Nov 1, 1946·The Journal of Organic Chemistry·E SCHWENK, D PAPA
Jan 1, 1972·Methods in Enzymology·M H Klapper, I M Klotz

❮ Previous
Next ❯

Citations

Apr 26, 2012·Chemical Reviews·Clémence QueffélecBruno Bujoli
Jun 7, 2008·Chemical Communications : Chem Comm·Marc A Gauthier, Harm-Anton Klok
Mar 13, 2009·Physical Chemistry Chemical Physics : PCCP·David L Cheung, Alessandro Troisi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.