Protein transduction by lipidic peptide dendrimers

Journal of Pharmaceutical Sciences
Henry K BayeleAlexander T Florence

Abstract

We investigated the potential of a new family of lipidic peptide dendrimers in protein transduction into cultured cells. Dendrimer-protein interaction was determined by gel retardation assays using purified recombinant protein. To assess intracellular protein delivery, two marker proteins were used: recombinant firefly luciferase and a Cy3-labeled monoclonal antibody to the c-myc proto-oncogene. Protein delivery was determined by luciferase assays and fluorescence microscopy, respectively. While there was minimal delivery of luciferase or antibody in the absence of the dendrimers, the latter increased protein delivery substantially. Luciferase delivery was concentration and cell type-dependent; the efficiency of delivery also varied with the number of terminal amino groups on the dendrimers. In previous reports, we showed that these dendrimers could be used for gene and drug delivery; the data we report herein suggest that they may also be capable of intracellular protein delivery. This finding has important implications for the use of these dendrimers in protein therapeutics and vaccinology.

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Citations

Mar 28, 2009·International Journal of Molecular Sciences·Sandra VeldhoenTobias Restle
Aug 28, 2007·Journal of Pharmaceutical Sciences·Yiyun ChengTongwen Xu
Nov 13, 2010·Biomaterials·Anjali SharmaDusica Maysinger
May 5, 2010·Chemistry : a European Journal·Rami HouraniAshok Kakkar
Jan 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuting NiuChengzhong Yu
Dec 23, 2008·Chemical Reviews·Rakesh Kumar TekadeNarendra Kumar Jain
Jan 18, 2018·Advanced Drug Delivery Reviews·Abhirup MandalAshim K Mitra
Oct 20, 2011·Journal of the American Chemical Society·Xiaoxing SunBrian G Trewyn
Sep 27, 2019·Chemical Reviews·Rachit SapraV Haridas

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