Protein transfer-mediated surface engineering to adjuvantate virus-like nanoparticles for enhanced anti-viral immune responses

Nanomedicine : Nanotechnology, Biology, and Medicine
Jaina M PatelPeriasamy Selvaraj

Abstract

Recombinant virus-like nanoparticles (VLPs) are a promising nanoparticle platform to develop safe vaccines for many viruses. Herein, we describe a novel and rapid protein transfer process to enhance the potency of enveloped VLPs by decorating influenza VLPs with exogenously added glycosylphosphatidylinositol-anchored immunostimulatory molecules (GPI-ISMs). With protein transfer, the level of GPI-ISM incorporation onto VLPs is controllable by varying incubation time and concentration of GPI-ISMs added. ISM incorporation was dependent upon the presence of a GPI-anchor and incorporated proteins were stable and functional for at least 4weeks when stored at 4°C. Vaccinating mice with GPI-granulocyte macrophage colony-stimulating factor (GM-CSF)-incorporated-VLPs induced stronger antibody responses and better protection against a heterologous influenza virus challenge than unmodified VLPs. Thus, VLPs can be enriched with ISMs by protein transfer to increase the potency and breadth of the immune response, which has implications in developing effective nanoparticle-based vaccines against a broad spectrum of enveloped viruses. The inherent problem with current influenza vaccines is that they do not generate effective cross-protection ag...Continue Reading

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Citations

Jan 20, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Karin L LeeNicole F Steinmetz
Apr 9, 2016·Expert Review of Vaccines·Fu-Shi QuanSang-Moo Kang
Aug 21, 2016·Journal of Lipid Research·Susanne HeiderChristoph Metzner
Nov 22, 2017·PeerJ·Hui Kian OngKok Lian Ho
Jan 7, 2020·Expert Review of Vaccines·Fu-Shi QuanSang-Moo Kang
Nov 7, 2017·Biochimica Et Biophysica Acta. Biomembranes·Susanne HeiderChristoph Metzner

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