Protein transfer through polyacrylamide hydrogel membranes polymerized in lyotropic phases

Biomacromolecules
Michael J MonteiroLi Xie

Abstract

A way to control the average pore size in cross-linked polyacrylamide-based membranes is by altering the ratio of cross-linker to acylamide monomer. Larger pore sizes are prepared with a minimum amount of cross-linker, resulting in membranes that are mechanically weak and have short lifetimes. The aim of this study was to prepare cross-linked polyacrylamide membranes with large pore sizes and with good mechanical integrity. The methodology was to carry out the polymerization in a template, formed from the self-aggregation of surfactant. Two surfactant templates were used, and their pore size was examined with proteins of different sizes. The surfactants chosen for this study were sodium dodecyl sulfate (SDS, ionic surfactant) and TERIC BL8 (nonionic surfactant), both of which have very different aggregation properties. The data showed that at 10% and greater of TERIC BL8, a very different and open gel structure is formed, in which the pore size was significantly increased. SDS seemed to have little effect on the pore size. The data suggests that the gel structures for both surfactants up to 4% (w/v) are similar and micellular, because SDS is known to favor a micelle structure. Above 4% (w/v), TERIC BL8 then goes through a chang...Continue Reading

References

Jul 1, 1994·Electrophoresis·Z S HorvathJ Margolis
Mar 1, 1997·Electrophoresis·G L CorthalsA A Gooley
Feb 16, 2000·Journal of Chromatography. a·D B RylattC H Nair

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Citations

Jun 20, 2006·Journal of Colloid and Interface Science·Edith C RojasKyriakos D Papadopoulos
Oct 2, 2014·ACS Applied Materials & Interfaces·Bijay Prakash TripathiManfred Stamm
Nov 26, 2009·The American Journal of Chinese Medicine·Jin-Xia YangXue-Mei Wang

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