PMID: 9451453Feb 6, 1998Paper

Protein translocation functions of Escherichia coli SecY: in vitro characterization of cold-sensitive secY mutants

Biochimie
T TauraK Ito

Abstract

Protein translocation across the plasma membrane of E coli is facilitated by Sec factors, including the membrane-embedded SecYEG subunit and the SecA ATPase. Although there is complete agreement that SecA is essential for protein translocation, some publications question the essentialness of SecY. We previously isolated a number of cold-sensitive mutants of secY and characterized their in vivo phenotypes. In this study, we characterized membrane vesicles prepared from these mutants with respect to their in vitro activities to support protein translocation and to activate the SecA ATPase. These studies revealed several single amino acid alterations that abolish these in vitro activities of membrane vesicles. In particular, several mutations in the two most carboxy-terminal cytoplasmic domains of SecY prevented SecA from functioning as the translocation ATPase. A number of mutants showed strong correlations between in vivo protein export ability, in vitro translocation activity and in vitro translocation ATPase activity, substantiating the importance of SecY in vivo and in vitro. A few other mutants were affected in only one or two aspects of these properties, suggesting that they were differentially affected in some substeps of ...Continue Reading

References

Nov 15, 1979·Analytical Biochemistry·P A LanzettaO A Candia
Mar 16, 1990·Biochemical and Biophysical Research Communications·Y Akiyama, K Ito
Dec 1, 1990·Journal of Bacteriology·T BabaK Ito
Jun 6, 1994·FEBS Letters·B JungnickelE Hartmann
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Watanabe, G Blobel
Nov 22, 1996·The Journal of Biological Chemistry·W Wickner, M R Leonard
Nov 29, 1996·The Journal of Biological Chemistry·A K Corsi, R Schekman
Apr 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K Ito
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Louise H BoyleJohn Trowsdale

❮ Previous
Next ❯

Citations

Oct 13, 2004·Biochemistry·Ligong WangDebra A Kendall
Feb 13, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·G MatsumotoK Ito
Aug 10, 2000·Molecular Microbiology·E H Manting, A J Driessen
Aug 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Akiko MurakamiKoreaki Ito
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hiroyuki Mori, Koreaki Ito
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·G MatsumotoK Ito
Feb 15, 2011·The Journal of Biological Chemistry·Sanchaita Das, Donald B Oliver
Mar 27, 2002·Journal of Bacteriology·Kazuhiko ChibaKoreaki Ito
Sep 11, 2002·FEBS Letters·Eli O van der SluisArnold J M Driessen
Aug 8, 2006·Journal of Molecular Biology·Eli O van der SluisArnold J M Driessen
Nov 18, 2004·Biochimica Et Biophysica Acta·Andreas K J VeenendaalArnold J M Driessen
Oct 3, 2006·Biochimica Et Biophysica Acta·Sharyn L Rusch, Debra A Kendall
Aug 10, 2000·The Journal of Cell Biology·H G Koch, M Müller
Sep 13, 2000·The Journal of Biological Chemistry·H NakatogawaK Ito
May 21, 2003·Molecular Genetics and Genomics : MGG·N ShimokawaK Ito
Sep 28, 2002·The Journal of Biological Chemistry·Hiroyuki MoriKoreaki Ito
Apr 15, 2021·Structure·Srinath KrishnamurthyAnastassios Economou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.