PMID: 11607171Apr 1, 1991Paper

Protein turnover as a component in the light/dark regulation of phosphoenolpyruvate carboxylase protein-serine kinase activity in C4 plants

Proceedings of the National Academy of Sciences of the United States of America
J A JiaoRaymond Chollet

Abstract

Maize leaf phosphoenolpyruvate carboxylase [PEPC; orthophosphate:oxaloacetate carboxy-lyase (phosphorylating), EC 4.1.1.31] protein-serine kinase (PEPC-PK) phosphorylates serine-15 of its target enzyme, thus leading to an increase in catalytic activity and a concomitant decrease in malate sensitivity of this cytoplasmic C4 photosynthesis enzyme in the light. We have recently demonstrated that the PEPC-PK activity in maize leaves is slowly, but strikingly, increased in the light and decreased in darkness. In this report, we provide evidence that cycloheximide, an inhibitor of cytoplasmic protein synthesis, when fed to detached leaves of C4 monocots (maize, sorghum) and dicots (Portulaca oleracea) in the dark or light, completely prevents the in vivo light activation of PEPC-PK activity regardless of whether the protein kinase activity is assessed in vivo or in vitro. In contrast, chloramphenicol, an inhibitor of protein synthesis in chloroplasts, has no effect on the light activation of maize PEPC-PK. Similarly, treatment with cycloheximide did not influence the light activation of other photosynthesis-related enzymes in maize, including cytoplasmic sucrose-phosphate synthase and chloroplast stromal NADPH-malate dehydrogenase an...Continue Reading

References

May 1, 1989·Archives of Biochemistry and Biophysics·J L HuberT H Nielsen
Mar 1, 1988·Archives of Biochemistry and Biophysics·J A Jiao, R Chollet
Sep 15, 1988·Biochemical and Biophysical Research Communications·C EchevarríaP Gadal
Dec 1, 1990·Plant Physiology·R J Budde, D D Randall

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Citations

Aug 31, 2001·Biochemical and Biophysical Research Communications·N BakrimR Chollet
Feb 1, 1994·Photosynthesis Research·A V RajagopalanA S Raghavendra
Jun 5, 2003·Archives of Biochemistry and Biophysics·Hugh G Nimmo
Feb 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·A C L NobreH S A Monteiro
Jul 18, 2002·Physiologia Plantarum·María V LaraCarlos S Andreo
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·J HartwellH G Nimmo
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Raymond CholletMarion H. O'Leary
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Jen Sheen
Jan 11, 2007·Médecine sciences : M/S·Jean VidalCristina Echevarría
Aug 24, 2006·The Plant Journal : for Cell and Molecular Biology·Matt ShentonHugh G Nimmo
Jul 10, 2007·FEBS Letters·José Antonio MonrealSofía García-Mauriño
Jul 1, 2001·The New Phytologist·Hugh G NimmoMalcolm B Wilkins

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