Protein tyrosine kinase activity and the influence of gender in phagocytosis and tumor necrosis factor secretion in alveolar macrophages and lung-recruited neutrophils

Shock
J J Spitzer, P Zhang

Abstract

The role of tyrosine phosphorylation in endotoxin-induced phagocytic and tumor necrosis factor secretory responses was studied in rat alveolar macrophages and lung-recruited neutrophils. Exploration of sexual dimorphism in some aspects of these functions was also a specific aim. Male and female rats were injected intratracheally with endotoxin or saline. Two and a half hours later the animals were subjected to bronchoalveolar lavage, and alveolar macrophages and lung-recruited neutrophils were isolated. Circulating neutrophils of endotoxin-treated rats were also isolated at this time. Phagocytosis and CD11b/c and CD18 expression were measured by flow cytometry; tumor necrosis factor was measured with a cytotoxicity assay. Using the protein tyrosine kinase inhibitor AG126 and phosphotyrosine immunoblotting, we demonstrated that tyrosine phosphorylation is an important signaling pathway in the activation of these cells by endotoxin and that it is coupled to phagocytosis and tumor necrosis factor secretion, but not to beta 2 integrin expression. Conditioned medium of alveolar macrophages of endotoxin-injected rats upregulates phagocytosis by blood neutrophils of naive rats and this upregulating activity is tyrosine phosphorylation...Continue Reading

Citations

Nov 30, 2002·Alcohol·Judy A SpitzerSteve Nelson
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rabindra TirouvanziamLeonard A Herzenberg
Sep 1, 2007·Antioxidants & Redox Signaling·Ren-Feng Guo, Peter A Ward
Jun 11, 2011·Journal of Inflammation·René RobertJean-Michel Goujon
May 5, 2006·Medicine and Science in Sports and Exercise·Adrienne S BrownEugene P Mayer
Nov 13, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·David H RobertsLeo C Ginns
Mar 4, 2015·Glia·Christiane MenzfeldUwe-Karsten Hanisch
Sep 5, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Adalet DemirDirk Van Raemdonck
Mar 22, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·O LortholaryF Dromer

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