PMID: 8604027Mar 1, 1996Paper

Protein tyrosine kinases in activation signal of human basophils through the immunoglobulin E receptor type l

Journal of Leukocyte Biology
M BenhamouM Arock

Abstract

Human basophils activated through high-affinity immunoglobulin E (IgE) receptors (Fc epsilon RI) are involved in the late phase of the allergic reaction. To investigate the possible involvement of protein-tyrosine kinases in this activation we used human acute basophilic leukemia (ABL) cells in culture as well as a pure population of normal basophils in vitro-derived from human bone marrow precursor cells (HBMB). ABL cells were 50-80% basophils at various stages of maturation as assessed by staining, morphology, ultrastructure, and flow cytometry analysis, and only basophils in ABL cells expressed Fc epsilon RI. Aggregation of Fc epsilon RI by IgE and anti-IgE, IgE and antigen, or anti-Fc epsilon RI monoclonal antibodies on ABL cells or on HBMB, led to increased tyrosine phosphorylation of 120-, 100-, 80-, 72-, 50- to 65-, and 38-kDa substrates. Tyrosine phosphorylations in ABL cells were in basophils because 1) they were detected after a 5-s stimulation, 2) they were observed under conditions where mediator release is minimal, i.e., in the absence of extracellular calcium, 3) hapten addition during antigen stimulation resulted in almost total disappearance of tyrosine phosphorylations within 30 s. There was correlation between...Continue Reading

Citations

Jun 9, 2012·The Journal of Biological Chemistry·Youngmi KimDooil Jeoung
Aug 30, 2000·International Journal of Immunopharmacology·A TedeschiA Miadonna
Aug 23, 2001·International Immunopharmacology·M BidriM Arock
Sep 2, 1998·The Journal of Allergy and Clinical Immunology·C L KepleyJ M Oliver

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