Protein tyrosine-O-sulfation analysis by exhaustive product ion scanning with minimum collision offset in a NanoESI Q-TOF tandem mass spectrometer

Analytical Chemistry
Mogjiborahman SalekWolf D Lehmann

Abstract

Tyrosine-O-sulfated peptides were studied by nanoESI Q-TOF mass spectrometry and were found to exhibit an abundant loss of SO3 in positive ion mode under the usually nonfragmenting conditions of survey spectrum acquisition. A new strategy for the detection of tyrosine-O-sulfated peptides in total protein digests was designed based on exhaustive product ion scanning at the collision offset conditions typical for the recording of survey spectra (minimum collision offset). From these data, Q-TOF neutral loss scans for loss of 80/z and Q-TOF precursor ions scans were extracted. The specificity of this approach for analysis of tyrosine-O-sulfation was tested using a tryptic digest of bovine serum albumin spiked with sulfated hirudin (1:1 and 1000:1 molar ratio of BSA to sulfated hirudin, respectively) and using an in-solution digest of the recombinant extracellular domain of thyroid stimulating hormone receptor (ECD-TSHr). For both examples, the combination of in silico neutral loss scans for 80/z and subsequent in silico precursor ion scans resulted in a specific identification of sulfated peptides. In the analysis of recombinant ECD-TSHr, a doubly sulfated peptide could be identified in this way. Surprisingly, approximately 1/4 of...Continue Reading

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Citations

Aug 11, 2007·Analytical and Bioanalytical Chemistry·Chien-Wen HungWolf D Lehmann
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Shannon L Cook, Glen P Jackson
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Marina Edelson-AverbukhWolf D Lehmann
Jun 15, 2007·Nature Methods·Yonghao YuJulie A Leary
Dec 3, 2008·The Journal of Biological Chemistry·Adam J HoffhinesKevin L Moore
Apr 10, 2007·Biomolecular Engineering·Joerg Reinders, Albert Sickmann
Sep 6, 2006·Biochimica Et Biophysica Acta·Flavio MonigattiHanno Steen
Sep 15, 2005·Analytical Biochemistry·Yukari AmanoYoshikatsu Matsubayashi
Jan 31, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuh-Shyong YangYi-Chih Mao
Jun 2, 2006·Electrophoresis·Mikko J LammiAnitta Mahonen
Nov 15, 2005·Mass Spectrometry Reviews·Mirko Glinski, Wolfram Weckwerth
Sep 10, 2008·Angewandte Chemie·Navin VaradarajanBrent L Iverson
Nov 1, 2006·Analytical Chemistry·Haichuan Liu, Kristina Håkansson
Oct 27, 2010·Journal of Proteome Research·Shen NiuYixue Li
Jul 4, 2013·Journal of Proteome Research·Jakob BunkenborgHenrik Molina

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