Protein tyrosine phosphatases mediate cell readhesion in alveolar epithelial cells mechanically separated from in vitro matrix

American Journal of Respiratory Cell and Molecular Biology
B L OliverM A Matthay

Abstract

Alveolar epithelial type II cells are the progenitor cells for restoring the alveolar epithelial barrier after acute lung injury. During repair of lung injury, the alveolar epithelial type II cells reepithelialize denuded air spaces, a process that involves breaking and reforming cell adhesions. A novel technique of mechanical separation of cultured alveolar epithelial cells from in vitro matrix was used to examine the intracellular signals that result when alveolar epithelial cell adhesions are broken. The results show that the tyrosine phosphorylation levels of focal adhesion kinase, paxillin, and pp60(src) decreased immediately after mechanical separation of the cells. Levels returned to nearly normal by 24 h after mechanical separation. Paxillin and pp60(scr) coprecipitated with focal adhesion kinase regardless of their phosphorylation state. Interestingly, the tyrosine phosphorylation level of the mitogen-activated protein kinase, p42(erk2), increased 15 min after mechanical separation. Preincubation of cell monolayers with phenylarsine oxide, a protein tyrosine phosphatase inhibitor, blocked the decrease in tyrosine phosphorylation levels of focal adhesion kinase, paxillin and pp60(src). Phenylarsine oxide incubation also...Continue Reading

Citations

Oct 6, 2000·Clinics in Chest Medicine·D H Ingbar
Aug 15, 2000·Molecular Carcinogenesis·G RamakrishnaL M Anderson
Jul 3, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Leena P DesaiChristopher M Waters
Jan 17, 2020·Scientific Reports·Mingxing FangZhiyong Wang
May 4, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Thomas GeiserV Courtney Broaddus

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