PMID: 7537105Apr 13, 1995Paper

Protein tyrosine phosphorylation in human platelets induced by interaction between glycoprotein Ib and von Willebrand factor

Biochimica Et Biophysica Acta
Y OzakiS Kume

Abstract

The interaction between von Willebrand factor (vWF) and glycoprotein Ib (GPIb) induced by ristocetin or botrocetin resulted in associated platelet aggregation, protein tyrosine phosphorylation (PTP) of a 64 kDa protein, as detected by a monoclonal antibody against phosphotyrosine (PY-20), and intracellular Ca2+ elevation that is largely dependent upon Ca2+ influx in human platelets. It is of interest that 75-80, 97 and 125 kDa proteins which are strongly tyrosine-phosphorylated in platelet activation induced by thrombin and other agonists were not detected. Neither vWF nor a coaggregating agent (ristocetin or botrocetin) alone induced aggregation, [Ca2+]i elevation or the 64 kDa PTP. NMC-4, an antibody which inhibits both ristocetin- or botrocetin-induced vWF binding to GPIb, abolished the appearance of the 64 kDa PTP as well as other responses, suggesting that it is specifically induced by the GPIb-vWF interaction. Aspirin, or ONO-3708, a competitive inhibitor of thromboxane A2, did not modify the 64 kDa PTP, while [Ca2+]i elevation was moderately suppressed. Depletion of extracellular Ca2+ or RGD peptides suppressed neither the 64 kDa PTP nor aggregation. H-7, a protein kinase C inhibitor, did not inhibit the 64 kDa PTP, whil...Continue Reading

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Citations

Jan 1, 1997·The International Journal of Biochemistry & Cell Biology·R K AndrewsM C Berndt
Sep 22, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·M Keegan DelaneyXiaoping Du
Jul 17, 2013·Journal of Thrombosis and Haemostasis : JTH·Y OzakiO Inoue
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·Y OzakiM C Berndt
Feb 15, 2005·Journal of Photochemistry and Photobiology. B, Biology·Zhiwen HuZengliang Yu
Jan 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M D Sjaastad, W J Nelson
Aug 2, 2003·Blood·Vipul RathorePeter J Newman
Apr 3, 2004·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Hongyan XiaoDaniel G Remick
Jun 10, 2017·International Journal of Molecular Sciences·Rachit BadoliaSatya P Kunapuli
Jul 15, 2004·Journal of Cell Science·Jonathan M Gibbins
Sep 24, 2004·Cellular Signalling·Ilaria CanobbioMauro Torti

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