Protein tyrosine phosphorylation influences adhesive junction assembly and follicular organization of cultured thyroid epithelial cells

Endocrinology
A S YapS W Manley

Abstract

The follicular histoarchitecture of the thyroid forms the anatomical basis for thyroid physiology and is commonly disturbed in diseases of the thyroid. We have used cultured porcine thyroid cells to study thyroid epithelial morphogenesis and its regulation. When cultured in the presence of TSH, freshly isolated thyroid cells reorganize to form follicles within three-dimensional cell aggregates. However, when established follicles are washed into TSH-free medium, thyroid cells spread and migrate to convert follicles into confluent epithelioid monolayers, activating morphogenetic mechanisms, such as cell locomotility, that may be relevant to thyroid inflammation and tumor invasiveness. The phenomenon of follicle to monolayer conversion, therefore, provides an opportunity to identify morphogenetic mechanisms that 1) must be tonically inhibited to maintain follicular organization and 2) may contribute to pathogenetic disturbances of follicular architecture when functioning aberrantly. In this study we found that follicle to monolayer conversion is associated with an increase in cellular phosphotyrosine. This was particularly evident at nascent focal adhesions (cell-substrate adhesive junctions) and later at cell-cell junctions. Foc...Continue Reading

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Citations

Jul 14, 2011·Journal of Cellular Physiology·Radhika SusarlaMargaret C Eggo
Feb 8, 2003·International Journal of Surgical Pathology·P SmythE C Sweeney
Aug 9, 2001·International Journal of Surgical Pathology·O. M. SheilsE. C. Sweeney
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Aug 15, 2002·American Journal of Physiology. Cell Physiology·Xin GuoJian-Ying Wang
May 23, 2015·Cell Biology International·Irina B AlievaIvan A Vorobjev

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