Abstract
Proteinase 3 (PR3) is the autoantigen in granulomatosis with polyangiitis, an autoimmune necrotizing vasculitis associated with anti-neutrophil cytoplasmic antibodies (ANCAs). Moreover, PR3 is a serine protease whose membrane expression can potentiate inflammatory diseases such as ANCA-associated vasculitis and rheumatoid arthritis. During apoptosis, PR3 is co-externalized with phosphatidylserine (PS) and is known to modulate the clearance of apoptotic cells through a calreticulin (CRT)-dependent mechanism. The complement protein C1q is one mediator of efferocytosis, the clearance of altered self-cells, particularly apoptotic cells. Since PR3 and C1q are both involved in the clearance of apoptotic cells and immune response modulation and share certain common ligands (i.e., CRT and PS), we examined their possible interaction. We demonstrated that C1q binding was increased on apoptotic rat basophilic leukemia (RBL) cells that expressed PR3, and we demonstrated the direct interaction between purified C1q and PR3 molecules as shown by surface plasmon resonance. To better understand the functional consequence of this partnership, we tested C1q-dependent phagocytosis of the RBL cell line expressing PR3 and showed that PR3 impaired C1...Continue Reading
References
May 1, 1997·Clinical and Experimental Immunology·U KishoreP Eggleton
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J S NavratilJ M Ahearn
Oct 2, 2002·The Journal of Biological Chemistry·Véronique Witko-SarsatBéatrice Descamps-Latscha
Oct 25, 2002·Immunobiology·Marina Botto, Mark J Walport
Oct 22, 2005·Cell·Shyra J GardaiPeter M Henson
Aug 23, 2007·Blood·Chahrazade KantariVéronique Witko-Sarsat
Dec 14, 2007·Proteins·Eric HajjarNathalie Reuter
Feb 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helena PaïdassiPhilippe Frachet
Oct 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Deborah A FraserAndrea J Tenner
Apr 29, 2010·The FEBS Journal·Eric HajjarNathalie Reuter
Mar 1, 2011·Journal of Molecular Biology·Helena PaïdassiPhilippe Frachet
Apr 27, 2012·Frontiers in Immunology·Berhane GhebrehiwetEllinor I B Peerschke
May 9, 2012·Frontiers in Immunology·Amiram Ariel, Charles N Serhan
Jul 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie GabilletVéronique Witko-Sarsat
Jan 4, 2013·Cold Spring Harbor Perspectives in Biology·Amelia Hochreiter-Hufford, Kodi S Ravichandran
Apr 24, 2013·Clinical and Experimental Immunology·L A TrouwR E M Toes
Feb 1, 2014·Nature Reviews. Immunology·Ivan K H PoonKodi S Ravichandran
Feb 22, 2014·Journal of Innate Immunity·Mélanie VerneretPhilippe Frachet
Jul 9, 2014·Nature Reviews. Rheumatology·J Charles Jennette, Ronald J Falk
May 24, 2015·Immunobiology·Diane Scott, Marina Botto
Oct 6, 2015·The Journal of Clinical Investigation·Arnaud MilletVéronique Witko-Sarsat
Mar 11, 2016·The Journal of Biological Chemistry·Katherine R MartinVéronique Witko-Sarsat
Apr 3, 2016·Seminars in Immunology·Nathalie ThieblemontVéronique Witko-Sarsat
Jun 28, 2016·European Journal of Immunology·Amiram Ariel, Kodi S Ravichandran
May 27, 2017·Journal of Leukocyte Biology·Katherine R Martin, Véronique Witko-Sarsat
Sep 8, 2017·Frontiers in Immunology·Rim OsmanPhilippe Frachet
Citations
Feb 23, 2020·Frontiers in Immunology·Zhilong JiangLei Zhu
Jul 25, 2018·European Journal of Clinical Investigation·Nathalie ThieblemontAmiram Ariel
Jul 7, 2020·Frontiers in Pharmacology·Hiba YaseenAmiram Ariel
Nov 22, 2018·Current Opinion in Rheumatology·Maria A C Wester TrejoIngeborg M Bajema
Oct 21, 2020·PloS One·Emel AkgunAhmet Tarik Baykal
Jan 12, 2021·Vascular·Dobroslav KyurkchievEkaterina Ivanova-Todorova
Dec 29, 2020·Frontiers in Immunology·Lina J SuárezAdriana Rodríguez
Mar 9, 2021·Frontiers in Immunology·Jérôme GranelCyrille Hoarau
Jul 3, 2021·Rheumatology·Mohammed OsmanJan Willem Cohen Tervaert
Oct 28, 2021·Immunology·Apurwa Singhal, Sachin Kumar