PMID: 7016984Mar 1, 1981Paper

Proteinases in pathology. Usefulness of histochemical methods

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Z Lojda

Abstract

The usefulness of optimized and newly elaborated histochemical methods for proteinases is illustrated on two selected substances. DAP IV (Gly-Pro-MNA,FBB,pH 7.2) was discovered in 39% and DAP II (Lys-Ala-MNA,FBB,pH 5.5) in 60% of the lymphocytes of human peripheral blood (ly). The reaction product of such ly differs in quality and quantity. On the ultrastructural level, the reaction product of DAP IV (Gly-Pro-MNA,HNF) was found in cell membranes and lysosomes. Enzyme activity in other areas was probably suppressed during the preparation procedure. Although the number of ly revealed with Lys-Pro-MNA and Phe-Pro-MNA at pH 5.5 and with Lys-Pro-MNA at pH 7.2 is high, these substrates do not distinctly discriminate DAP IV and DAP II. DAP IV occurs exclusively in T lymphocytes. The number of DAP IV-positive ly was not decreased in patients with myelofibrosis, plasmacytoma, chronic granulocytic leukemia, or tricholeukemia. It was, however, greatly reduced in chronic lymphatic leukemia (CLL). In patients with malignant lymphomas other than CLL, ly presence is related to the stage of the disease. Decreased values indicate a more severe stage or a relapse. In the majority of patients with gastric cancer DAP IV-positive ly were decreased....Continue Reading

Citations

Feb 13, 2001·Microscopy Research and Technique·J Vorísek
Jan 1, 1981·Histochemistry·S Gutschmidt, R Gossrau
Jul 1, 1984·Felʹdsher i akusherka·E V Vinogradov, N F Kanaev
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·A C FellerM R Parwaresch
Oct 1, 1985·The Histochemical Journal·Z Lojda
May 1, 1983·The Histochemical Journal·Z Lojda, E Havránková
Apr 1, 1984·The Histochemical Journal·Z LojdaV Synková
May 1, 1985·The Histochemical Journal·R GossrauR Graf
May 1, 1985·The Histochemical Journal·Z Lojda, E Benesová
Jul 1, 1985·The Histochemical Journal·R Gossrau
Sep 1, 1992·The Histochemical Journal·R E SmithE A Elder
Sep 1, 1989·The Histochemical Journal·M Ruhnke, R Gossrau
Jun 27, 2009·Cancer Immunology, Immunotherapy : CII·Oscar J CorderoMontserrat Nogueira
Jun 13, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y KasaharaF Chisari
Jan 1, 1989·Advances in Enzyme Regulation·W ReutterR Gossrau
Jan 1, 1987·Pharmacology & Therapeutics·R J MorinS K Peng
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·M Klobusická
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·J Vorísek
May 17, 2005·Brain Research. Molecular Brain Research·V MaresA Sedo
Dec 1, 1994·Acta histochemica·L E De Bault, A Mitro
Jan 1, 1991·Acta histochemica·D StillerC August

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