PMID: 7029274Sep 1, 1981Paper

Proteinases of Leishmania mexicana amastigotes and promastigotes: analysis by gel electrophoresis

Molecular and Biochemical Parasitology
M J North, G H Coombs

Abstract

The proteinases of Leishmania mexicana mexicana amastigotes and promastigotes have been analysed by electrophoresis on polyacrylamide gels containing denatured haemoglobin. Eleven bands of activity were detected indicating multiple proteinases. These were significant quantitative and qualitative differences between the proteinases of the two developmental forms. Four, B-E, were present in both forms but were of much higher activity in the amastigote. There were two major activities in promastigotes, A and D. The other proteinases, F-K, were of lower activity; I and K were not detected in promastigotes. All proteinases were active optimally at pH 4.0. Most of them, including the major proteinases A-E, were thiol proteinases since they were stimulated by 1 mM dithiothreitol and were sensitive to inhibitors such as HgCl2, leupeptin, antipain and iodoacetic acid.

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Citations

Sep 17, 1982·Molecular and Cellular Biochemistry·P RautenbergH J Risse
Jan 1, 1982·Transactions of the Royal Society of Tropical Medicine and Hygiene·G H CoombsJ Capaldo
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·A R Bogliolo, D G Godfrey
Nov 1, 1981·Molecular and Biochemical Parasitology·D T HartG H Coombs
Jun 1, 1988·Molecular and Biochemical Parasitology·S C AlfieriM Rabinovitch
Feb 1, 1990·Molecular and Biochemical Parasitology·A E EakinJ H McKerrow
Feb 1, 1990·Molecular and Biochemical Parasitology·S Greig, F Ashall
Sep 1, 1990·Molecular and Biochemical Parasitology·C D Robertson, G H Coombs
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H F Hassan, G H Coombs
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H F Hassan, G H Coombs
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M F Pupkis, G H Coombs
Apr 23, 2003·International Journal for Parasitology·Fernanda LasakosvitschClara Lúcia Barbiéri
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Fong, K P Chang
Mar 1, 1992·The Journal of protozoology·P ManningE L Jarroll
Mar 1, 1993·The Journal of Eukaryotic Microbiology·A A PanD McMahon-Pratt
Aug 13, 2008·Molecular and Biochemical Parasitology·Marcel Marín-VillaYara M Traub-Csekö
Dec 1, 1982·Experimental Parasitology·D T Hart, G H Coombs
Aug 1, 1986·Experimental Parasitology·M F PupkisG H Coombs
Feb 1, 1989·Experimental Parasitology·D F HareD G Lindmark
Feb 1, 1984·The Journal of protozoology·J C MeadeA J Mukkada
Feb 15, 2011·Molecular and Biochemical Parasitology·Ana BrennandPaul A M Michels
Jul 1, 1992·FEMS Microbiology Letters·C D Robertson, G H Coombs
Feb 1, 1984·Annals of Tropical Medicine and Parasitology·G H Coombs, J Baxter
Aug 1, 1985·Annals of Tropical Medicine and Parasitology·G H Coombs, B E Sanderson
Apr 1, 1985·Experimental Parasitology·H F Hassan, G H Coombs
Feb 1, 1984·Analytical Biochemistry·P J Kelleher, R L Juliano
Apr 1, 1997·Antimicrobial Agents and Chemotherapy·H L CallahanM Grogl
Aug 23, 2021·Bioorganic & Medicinal Chemistry·Wagner A S JudiceTiago Rodrigues

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