Proteinases secreted by Fasciola hepatica: time course of the inhibitory effect of serum from experimentally infected rabbits demonstrated by gelatin-substrate polyacrylamide gel electrophoresis

Journal of Helminthology
L PiacenzaC Carmona

Abstract

Fasciola hepatica secretes proteolytic enzymes to aid it to penetrate and migrate through the host tissues. Two of these proteinases have been purified and shown to be cathepsin L-like, and are termed, CL1 (27.5 kD) and CL2 (29 kD). The immunogenicity of these proteinases was investigated over the course of an experimental infection and following drug treatment. Four groups of rabbits were studied: group 1: orally infected with 50 metacercariae; group 2: infected and treated 8 weeks after infection; group 3: infected, treated at week 8 and reinfected at week 13 and group 4: non-infected control group. Sera were collected weekly from each group until week 20 postinfection. CL1 and CL2 were incubated with the different sera and then analysed by gelatin substrate polyacrylamide gel electrophoresis (GS-PAGE). Analysis of groups 1, 2 and 3 showed that CL1 and CL2 neutralizing antibodies appear at week 5 post-infection. In group 1, these remained throughout the 20 weeks of infection. In group 2, neutralizing antibodies disappeared at week 13, that is, 5 weeks after anti-Fasciola treatment. In group 3, CL1 and CL2 neutralizing antibodies disappeared at week 13 but reappeared by week 15, that is 2 weeks after reinfection. Pooled sera f...Continue Reading

References

Nov 1, 1991·International Journal for Parasitology·P J SharpG S Cobon
Apr 1, 1990·The American Journal of Tropical Medicine and Hygiene·C L ChappellA M Deelder
Jun 15, 1989·Molecular and Biochemical Parasitology·J P Dalton, M Heffernan
Apr 1, 1988·Molecular and Biochemical Parasitology·D P Knox, M W Kennedy
May 2, 1980·European Journal of Pharmacology·M H YenH K Lee
Jan 1, 1995·Parasitology Research·C de Armas-SerraF Rodriguez-Caabeiro
Nov 1, 1993·Molecular and Biochemical Parasitology·A M SmithJ P Dalton
Jan 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·A Jakobs, J Piette
Dec 1, 1993·International Journal for Parasitology·A M SmithJ P Dalton

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