Protein/DNA interaction profiling reveals novel regulators of the pineal transcriptome.

Molecular and Cellular Endocrinology
J S Davies, D Carter

Abstract

The rat pineal gland transcriptome exhibits dynamic daily variation that reflects nocturnally restricted hormone production. Here we have used a protein/DNA interaction array to screen for day-night changes in DNA binding activity that are associated with transcriptional rhythms. Overall, 47 of 54 potential consensus binding sequence activities were detected, and of these, 29 (62%) were found to exhibit day:night differences in level. In addition to known, rhythmic pineal DNA binding activities (CRE and AP-1), multiple novel activities were observed including nocturnally elevated AP-2 consensus sequence binding activity. This array result was validated using conventional DNA binding assays, and we have also demonstrated AP-2beta and AP-2gamma proteins in the pineal gland, in addition to a nocturnally elevated AP-2alpha isoform. Our results have confirmed the presence of a complex assembly of transcriptional rhythms in the rat pineal gland and have provided details of more factors that contribute to this aspect of circadian neuroendocrine function.

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Citations

Dec 24, 2008·The Journal of Biological Chemistry·Michael J BaileyDavid C Klein
Mar 22, 2007·Trends in Endocrinology and Metabolism : TEM·Erik Maronde, Jörg H Stehle

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