Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures

Biofouling
Yi YangPaul G Rouxhet

Abstract

The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation. Different substrata (polystyrene, glass, steel) were conditioned and analyzed by X-ray photoelectron spectroscopy. Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account. Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%. There was no significant difference between the compositions of the adlayers formed from different conditioning solutions, except for the adlayers produced with tightly bound EPS extracted from D. alaskensis.

References

Feb 23, 2002·Science·Cynthia B WhitchurchJohn S Mattick
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Sep 29, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cynthia BosquillonRita Vanbever
Feb 24, 2007·Journal of Colloid and Interface Science·François AhimouPaul G Rouxhet
Nov 21, 2007·Journal of Colloid and Interface Science·J LandoulsiP G Rouxhet
Jun 6, 2008·Journal of Colloid and Interface Science·C M DekeyserP G Rouxhet
Aug 18, 2009·Journal of Visualized Experiments : JoVE·Nadine Dyballa, Sabine Metzger
Oct 10, 2009·Journal of Agricultural and Food Chemistry·V Aguié-BéghinR Douillard
Feb 23, 2010·Colloids and Surfaces. B, Biointerfaces·Cristèle J NonckremanChristine C Dupont-Gillain
Jul 6, 2010·Molecular Microbiology·Cécile BerneYves V Brun
May 20, 2011·Colloids and Surfaces. B, Biointerfaces·Hans-Curt Flemming
Nov 25, 2011·Colloids and Surfaces. B, Biointerfaces·Geelsu HwangYang Liu
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Erin S GloagCynthia B Whitchurch
Aug 3, 2014·Journal of Colloid and Interface Science·Yetioman TouréPaul G Rouxhet

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Citations

May 4, 2017·International Journal of Food Microbiology·Jiaqi LiXiaonan Lu
Jan 12, 2017·Cytotechnology·Jinying HeJing Yang
Sep 25, 2018·Environmental Microbiology Reports·Jorge AntunesVitor Vasconcelos
Sep 22, 2019·Journal of Applied Microbiology·T RamiresW P da Silva
Sep 2, 2020·Biointerphases·Elise GardinPhilippe Marcus
Mar 30, 2021·Biochemistry and Biophysics Reports·Ravneet ChugVinod Singh Gour
Aug 31, 2018·Environmental Science & Technology·Alexander T L VosshageFriederike Gabel
Feb 8, 2019·ACS Applied Materials & Interfaces·Markus StöcklWolfgang Sand
Nov 16, 2021·The Science of the Total Environment·Diana N Michler-KozmaFriederike Gabel

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Methods Mentioned

BETA
X-ray
electrophoresis

Software Mentioned

Casa
CasaXPS

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