Proteins encoded by human Down syndrome critical region gene 1-like 2 (DSCR1L2) mRNA and by a novel DSCR1L2 mRNA isoform interact with cardiac troponin I (TNNI3)

Gene
Silvia CanaiderPierluigi Strippoli

Abstract

Down syndrome critical region gene 1-like 2 (DSCR1L2) belongs to the human DSCR1-like gene family, which also includes DSCR1 and DSCR1L1. Both DSCR1 and DSCR1L1 proteins interact with calcineurin, a calcium/calmodulin-dependent phosphatase. To date, no interactor has been described for DSCR1L2. The aim of this work was to perform a first functional study of DSCR1L2 using yeast two-hybrid analysis conducted on a human heart cDNA library. Here, we report the interaction between DSCR1L2 and the human cardiac troponin I (TNNI3), the heart-specific inhibitory subunit of the troponin complex, a central component of the contractile apparatus. This interaction was confirmed by both yeast cotransformation and GST (glutathione-sepharose transferase) fusion protein assay. Moreover, a new DSCR1L2 mRNA isoform, generated by alternative splicing, was identified and cloned in different tissues: it lacks two central exons, encoding the most conserved domains among the DSCR1-like protein family. A quantitative relative reverse transcription-polymerase chain reaction (RT-PCR) assay showed that in heart tissue the normalized expression level ratio for DSCR1L2 and DSCR1L2-E2E5 mRNA isoforms is 3.5:1, respectively. The yeast cotransformation and GS...Continue Reading

References

Apr 1, 1978·The Biochemical Journal·P Cummins, S V Perry
Nov 1, 1991·Circulation Research·N M HunkelerA M Murphy
Nov 1, 1993·Trends in Biochemical Sciences·W R Engels
Jun 14, 1996·The Journal of Biological Chemistry·T MiyazakiH Seo
Sep 13, 2002·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Pierluigi StrippoliMaria Zannotti
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert L StrausbergUNKNOWN Mammalian Gene Collection Program Team
Jan 15, 2003·Biochemistry·Qiang LiXing-Zhen Chen
Jan 18, 2003·The Journal of Clinical Investigation·Jens MogensenWilliam J McKenna

❮ Previous
Next ❯

Citations

Jan 28, 2014·PloS One·Eva Serrano-CandelasMercè Pérez-Riba
Nov 21, 2007·Gene·Federica FacchinPierluigi Strippoli
Mar 19, 2015·Biochemical and Biophysical Research Communications·Eva Serrano-CandelasMercè Pérez-Riba
Feb 3, 2007·Biochimica Et Biophysica Acta·M Carme MuleroMercè Pérez-Riba
Jun 28, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kelvin J A DaviesXavier Estivill
Apr 18, 2021·Journal of the American Society of Nephrology : JASN·Brandon M LaneRasheed Gbadegesin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.