PMID: 8606787Nov 1, 1995Paper

Proteins functioning in synaptic transmission at the sensory to motor synapse of Aplysia

Neuropharmacology
P A SkehelK C Martin

Abstract

Over expression of Aplysia synaptotagmin in acutely dissected cholinergic neurons from the buccal ganglia, or in primary co-cultures of glutaminergic sensory neurons and motor neurons, causes a reduction synaptic transmission. Anti-sense oligonucleotide treatment of similar cultures produced an enhancement of synaptic transmission. The interaction between Aplysia VAMP/synaptobrevin and syntaxin is reconstructed using the yeast two hybrid system, and used to identify amino acid residues of VAMP/synaptobrevin that are required for this interaction. Point mutations around residue 50, close to the site of cleavage by botulinum toxins specifically disrupt the interaction with syntaxin. An additional VAMP/synaptobrevin binding protein, VAP33, is identified using the yeast two hybrid system. Intracellular injection of VAP33 specific antisera inhibits synaptic transmission in sensory-motor neuron co-cultures.

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Citations

Nov 12, 2010·Amino Acids·Yanwei SunGert Lubec
Jun 14, 2000·Current Opinion in Neurobiology·A T Brunger
Apr 12, 2001·Current Opinion in Structural Biology·A T Brunger
Aug 13, 2004·Brain Research. Molecular Brain Research·Sami H Jezzini, Leonid L Moroz
Jul 29, 2004·Molecular and Biochemical Parasitology·D E K Tarr, Alan L Scott
Jun 8, 2015·Current Pain and Headache Reports·Nada Hindiyeh, Sheena K Aurora
Nov 26, 2014·Cellular and Molecular Life Sciences : CMLS·Marion Weber-BoyvatVesa M Olkkonen
Dec 3, 2014·Experimental Cell Research·Marion Weber-BoyvatVesa M Olkkonen
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·A T Brunger
Apr 20, 2005·Trends in Parasitology·Deirdre E K Tarr, Alan L Scott
Apr 7, 2009·Neurobiology of Learning and Memory·Tony D Gover, Thomas W Abrams
Jul 31, 1999·The Journal of Cell Biology·L SoussanZ Elazar
Apr 1, 2016·Scientific Reports·Maryam ShahidUwe Strähle
Nov 17, 2004·The Journal of Biological Chemistry·Roy AmarilioSima Lev

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